Literature DB >> 16453881

Transpositional behavior of the maize En/Spm element in transgenic tobacco.

A Pereira1, H Saedler.   

Abstract

The transposable element Enhancer (En/Spm) of Zea mays encodes the functions necessary for transposition of members of the En-I family of elements. En constructs were introduced into tobacco and transposition was demonstrated by cloning and DNA sequence analysis. Transcriptional analysis of En transgenic plants revealed 2.5 and 6 kb En homologous transcripts similar in size to those found in maize, in addition to aberrant splice products. Progeny analysis of a primary transformant indicates En transpositional activity after meiosis. In these progeny somatic variegation, at the NPT II gene, was demonstrated to be caused by En excision. Two re-insertions of En were analyzed and found to reside in unique copy DNA while another was found in middle repetitive DNA.

Entities:  

Year:  1989        PMID： 16453881      PMCID： PMC400957          DOI： 10.1002/j.1460-2075.1989.tb03511.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  19 in total

1.  Intranuclear systems controlling gene action and mutation.

Authors:  B MCCLINTOCK
Journal:  Brookhaven Symp Biol       Date:  1956-02

2.  Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus.

Authors:  P Masson; R Surosky; J A Kingsbury; N V Fedoroff
Journal:  Genetics       Date:  1987-09       Impact factor: 4.562

3.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

4.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  Organization of the Tgm family of transposable elements in soybean.

Authors:  P R Rhodes; L O Vodkin
Journal:  Genetics       Date:  1988-10       Impact factor: 4.562

6.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

7.  Isolation of the transposable maize controlling elements Ac and Ds.

Authors:  N Fedoroff; S Wessler; M Shure
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

8.  TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

Authors:  A Gierl; S Lütticke; H Saedler
Journal:  EMBO J       Date:  1988-12-20       Impact factor: 11.598

9.  Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens.

Authors:  E Van Haute; H Joos; M Maes; G Warren; M Van Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota.

Authors:  M A Van Sluys; J Tempé; N Fedoroff
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  19 in total

1.  A two-component enhancer-inhibitor transposon mutagenesis system for functional analysis of the Arabidopsis genome.

Authors:  E Speulman; P L Metz; G van Arkel; B te Lintel Hekkert; W J Stiekema; A Pereira
Journal:  Plant Cell       Date:  1999-10       Impact factor: 11.277

2.  Survival of Tdc transposable elements of the En/Spm superfamily in the carrot genome.

Authors:  Y Itoh; M Hasebe; E Davies; J Takeda; Y Ozeki
Journal:  Mol Genet Genomics       Date:  2003-02-12       Impact factor: 3.291

3.  Transcription and somatic transposition of the maize En/Spm transposon system in rice.

Authors:  R Greco; P B F Ouwerkerk; A J C Taal; C Sallaud; E Guiderdoni; A H Meijer; J H C Hoge; A Pereira
Journal:  Mol Genet Genomics       Date:  2003-11-14       Impact factor: 3.291

4.  Functional analysis of the 3'-terminal sequence of the maize controlling element (Ac) by internal replacement and deletion mutagenesis.

Authors:  J H Zhou; A Myers; A G Atherly
Journal:  Genetica       Date:  1991       Impact factor: 1.082

Review 5.  The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.

Authors:  M A Haring; C M Rommens; H J Nijkamp; J Hille
Journal:  Plant Mol Biol       Date:  1991-03       Impact factor: 4.076

6.  Novel DNA structures resulting from dTam3 excision in tobacco.

Authors:  M A Haring; S Scofield; M J Teeuwen-de Vroomen; G S Leuring; H J Nijkamp; J Hille
Journal:  Plant Mol Biol       Date:  1991-11       Impact factor: 4.076

7.  Transposition of the maize activator element in transgenic rice plants.

Authors:  N Murai; Z J Li; Y Kawagoe; A Hayashimoto
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

Review 8.  Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.

Authors:  G G Simpson; W Filipowicz
Journal:  Plant Mol Biol       Date:  1996-10       Impact factor: 4.076

9.  The behaviour of the autonomous maize transposable element En/Spm in Arabidopsis thaliana allows efficient mutagenesis.

Authors:  E Wisman; G H Cardon; P Fransz; H Saedler
Journal:  Plant Mol Biol       Date:  1998-08       Impact factor: 4.076

10.  The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion.

Authors:  D Long; M Martin; E Sundberg; J Swinburne; P Puangsomlee; G Coupland
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205
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