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Literature DB >> 16453801

The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer.

J G Ellis¹, D J Llewellyn, J C Walker, E S Dennis, W J Peacock.

Abstract

A 176 bp DNA sequence lying upstream of the octopine synthase (ocs) promoter, previously shown to have enhancer-like properties in transgenic tobacco [Ellis et al. (1987) EMBO J., 6, 11-16], functions as an enhancer in protoplasts of Zea mays (a monocot plant) and Nicotiana plumbaginifolia (a dicotplant). We have characterized this element by transient expression assays using a linked alcohol dehydrogenase (Adh1) promoter from Z. mays and the chloramphenicol acetyltransferase coding sequences. The ocs sequence functions in both orientations but its enhancing activity is dependent upon its distance from the Adh1 promoter. Transient expression assays using deletion mutants and synthetic oligonucleotides show that a 16 bp palindrome ACGTAAGCGCTTACGT, contained within the 176 bp fragment, is essential and sufficient for enhancing activity in transient expression assays.

Entities: Chemical

Year: 1987 PMID： 16453801 PMCID： PMC553770 DOI： 10.1002/j.1460-2075.1987.tb02636.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

19 in total

1. Heat shock regulatory elements function as an inducible enhancer in the Xenopus hsp70 gene and when linked to a heterologous promoter.

Authors: M Bienz; H R Pelham
Journal: Cell Date: 1986-06-06 Impact factor: 41.582

2. Expression of genes transferred into monocot and dicot plant cells by electroporation.

Authors: M Fromm; L P Taylor; V Walbot
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

3. Structure and expression of an alcohol dehydrogenase 1 gene from Pisum sativum (cv. "Greenfeast").

Authors: D J Llewellyn; E J Finnegan; J G Ellis; E S Dennis; W J Peacock
Journal: J Mol Biol Date: 1987-05-05 Impact factor: 5.469

4. DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.

Authors: G C Grosveld; E de Boer; C K Shewmaker; R A Flavell
Journal: Nature Date: 1982-01-14 Impact factor: 49.962

5. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.

Authors: J Banerji; S Rusconi; W Schaffner
Journal: Cell Date: 1981-12 Impact factor: 41.582

6. Sequential expression of new gene programs in inducer T-cell clones.

Authors: G J Freeman; C Clayberger; R DeKruyff; D S Rosenblum; H Cantor
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

7. Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.

Authors: L Otten; H De Greve; J P Hernalsteens; M Van Montagu; O Schieder; J Straub; J Schell
Journal: Mol Gen Genet Date: 1981

8. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.

Authors: H De Greve; P Dhaese; J Seurinck; M Lemmers; M Van Montagu; J Schell
Journal: J Mol Appl Genet Date: 1982

9. Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.

Authors: M Fromm; P Berg
Journal: Mol Cell Biol Date: 1983-06 Impact factor: 4.272

10. The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.

Authors: C Koncz; H De Greve; D André; F Deboeck; M Van Montagu; J Schell
Journal: EMBO J Date: 1983 Impact factor: 11.598

52 in total

1. Multiple ocs-like elements required for efficient transcription of the mannopine synthase gene of T-DNA in maize protoplasts.

Authors: P C Fox; V Vasil; I K Vasil; W B Gurley
Journal: Plant Mol Biol Date: 1992-10 Impact factor: 4.076

The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer.

1. Heat shock regulatory elements function as an inducible enhancer in the Xenopus hsp70 gene and when linked to a heterologous promoter.

2. Expression of genes transferred into monocot and dicot plant cells by electroporation.

3. Structure and expression of an alcohol dehydrogenase 1 gene from Pisum sativum (cv. "Greenfeast").

4. DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.

5. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.

6. Sequential expression of new gene programs in inducer T-cell clones.

7. Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.

8. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.

9. Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.

10. The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.

1. Multiple ocs-like elements required for efficient transcription of the mannopine synthase gene of T-DNA in maize protoplasts.

2. Organization and functional analysis of three T-DNAs from the vitopine Ti plasmid pTiS4.

3. Functional properties of the anaerobic responsive element of the maize Adh1 gene.

4. Three distinct regulatory elements comprise the upstream promoter region of the nopaline synthase gene.

Review 5. Two-way chemical signaling in Agrobacterium-plant interactions.

6. Tissue-Specific Expression of as-1 in Transgenic Tobacco.

7. Transient gene expression in cassava using high-velocity microprojectiles.

8. pEmu: an improved promoter for gene expression in cereal cells.

Review 9. Designer promoter: an artwork of cis engineering.

10. Modular organization and development activity of an Arabidopsis thaliana EF-1 alpha gene promoter.