Literature DB >> 16453608

Cloning of the extra sex combs gene of Drosophila and its identification by P-element-mediated gene transfer.

E Frei1, S Baumgartner, J E Edström, M Noll.   

Abstract

A large region containing the extra sex combs (esc) gene of Drosophila has been cloned by microdissection from polytene chromosomes and chromosomal walking. Within this DNA, the segment comprising the esc gene has been narrowed down in several steps. First, a deletion of the esc gene, Df(2L)esc, defined a region of 380 kb. Then, a duplication carrying the esc gene, Dp(2;2)GYL, and a deletion not eliminating esc, Df(2L)prd, further reduced this region to 160 kb. Finally, because esc transcripts are expected to be present in follicles but absent in adult males, mapping of follicle and adult male transcripts limited the esc region to 10 kb. Rigorous proof that this DNA contained the esc gene was obtained by P-element-mediated transformation. We conclude that the 12 kb of DNA used for transformation include the esc gene as well as all flanking sequences required for its proper regulation in the female germ line.

Entities:  

Year:  1985        PMID： 16453608      PMCID： PMC554288          DOI： 10.1002/j.1460-2075.1985.tb03727.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  46 in total

1.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
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2.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

3.  Lambdoid phages that simplify the recovery of in vitro recombinants.

Authors:  N E Murray; W J Brammar; K Murray
Journal:  Mol Gen Genet       Date:  1977-01-07

4.  Different requirements for homeotic genes in the soma and germ line of Drosophila.

Authors:  P A Lawrence; P Johnston; G Struhl
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

5.  Role of the esc+ gene product in ensuring the selective expression of segment-specific homeotic genes in Drosophila.

Authors:  G Struhl
Journal:  J Embryol Exp Morphol       Date:  1983-08

6.  Developmental studies of lethality associated with the antennapedia gene complex in Drosophila melanogaster.

Authors:  R E Denell; K R Hummels; B T Wakimoto; T C Kaufman
Journal:  Dev Biol       Date:  1981-01-15       Impact factor: 3.582

7.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

8.  A gene that regulates the bithorax complex differentially in larval and adult cells of Drosophila.

Authors:  P W Ingham
Journal:  Cell       Date:  1984-07       Impact factor: 41.582

9.  Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.

Authors:  F Scalenghe; E Turco; J E Edström; V Pirrotta; M Melli
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  18 in total

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Authors:  H Sommerfelt; H M Grewal; M K Bhan
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2.  The Pax2 homolog sparkling is required for development of cone and pigment cells in the Drosophila eye.

Authors:  W Fu; M Noll
Journal:  Genes Dev       Date:  1997-08-15       Impact factor: 11.361

Review 3.  Polycomb group proteins--epigenetic repressors with emerging roles in melanocytes and melanoma.

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4.  Characterization of a pancreatic DNase from pyloric caeca of atlantic cod (Gadus morhua L.).

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5.  Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice.

Authors:  Liguo Zhang; Zhijun Cheng; Ruizhen Qin; Yang Qiu; Jiu-Lin Wang; Xiekui Cui; Lianfeng Gu; Xin Zhang; Xiuping Guo; Dan Wang; Ling Jiang; Chuan-yin Wu; Haiyang Wang; Xiaofeng Cao; Jianmin Wan
Journal:  Plant Cell       Date:  2012-11-13       Impact factor: 11.277

6.  Dorsoventral patterning in the Drosophila central nervous system: the intermediate neuroblasts defective homeobox gene specifies intermediate column identity.

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7.  Mutational and in vitro protein-binding studies on centromere DNA from Saccharomyces cerevisiae.

Authors:  R Ng; J Carbon
Journal:  Mol Cell Biol       Date:  1987-12       Impact factor: 4.272

8.  The instability of the TE-like mutation Dp(2:2)GYL of Drosophila melanogaster is intimately associated with the hobo element.

Authors:  P Hatzopoulos; M Monastirioti; G Yannopoulos; C Louis
Journal:  EMBO J       Date:  1987-10       Impact factor: 11.598

9.  The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo.

Authors:  T Berleth; M Burri; G Thoma; D Bopp; S Richstein; G Frigerio; M Noll; C Nüsslein-Volhard
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598

10.  Evidence that the Abdominal-B r element function is conferred by a trans-regulatory homeoprotein.

Authors:  M DeLorenzi; N Ali; G Saari; C Henry; M Wilcox; M Bienz
Journal:  EMBO J       Date:  1988-10       Impact factor: 11.598
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