Literature DB >> 16453475

A transformation system for the hypotrichous ciliate Stylonychia mytilus.

I U Wünning1, H J Lipps.   

Abstract

We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear ;gene-size' DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.

Entities:  

Year:  1983        PMID： 16453475      PMCID： PMC555354          DOI： 10.1002/j.1460-2075.1983.tb01653.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  24 in total

1.  Chromatin structure in the macronucleus of the ciliate Stylonychia mytilus.

Authors:  H J Lipps; A Nock; M Riewe; G Steinbrück
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

2.  An octamer of histones in chromatin and free in solution.

Authors:  J O Thomas; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

3.  A new dominant hybrid selective marker for higher eukaryotic cells.

Authors:  F Colbère-Garapin; F Horodniceanu; P Kourilsky; A C Garapin
Journal:  J Mol Biol       Date:  1981-07-25       Impact factor: 5.469

4.  In vitro aggregation of the gene-sized DNA molecules of the ciliate Stylonychia mytilus.

Authors:  H J Lipps
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

5.  DNase I hypersensitivity of the terminal inverted repeat DNA sequences in the macronucleus of the ciliate Stylonychia mytilus.

Authors:  H J Lipps; P Erhardt
Journal:  FEBS Lett       Date:  1981-04-20       Impact factor: 4.124

6.  Genetic apparatus of Stylonychia sp.

Authors:  D M Prescott; K G Murti; C J Bostock
Journal:  Nature       Date:  1973-04-27       Impact factor: 49.962

7.  Free genes for rRNAs in the macronuclear genome of the ciliate Stylonychia mytilus.

Authors:  H J Lipps; G Steinbrück
Journal:  Chromosoma       Date:  1978-10-20       Impact factor: 4.316

8.  Histone genes in macronuclear DNA of the ciliate Stylonychia mytilus.

Authors:  S M Elsevier; H J Lipps; G Steinbrück
Journal:  Chromosoma       Date:  1978-12-06       Impact factor: 4.316

9.  A DNA-mediated transformation system for Dictyostelium discoideum.

Authors:  K P Hirth; C A Edwards; R A Firtel
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

10.  RNA and macronuclear transcription in the ciliate Stylonychia mytilus.

Authors:  A Nock
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316
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  10 in total

1.  Gene dosage as a possible major determinant for equal expression levels of genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes octocarinatus.

Authors:  J Kaufmann; A Klein
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

2.  Transformation of Paramecium by microinjection of a cloned serotype gene.

Authors:  R Godiska; K J Aufderheide; D Gilley; P Hendrie; T Fitzwater; L B Preer; B Polisky; J R Preer
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

3.  Faithful expression of a heterologous gene carried on an artificial macronuclear chromosome in Euplotes crassus.

Authors:  J Bender; M Kämpfer; A Klein
Journal:  Nucleic Acids Res       Date:  1999-08-01       Impact factor: 16.971

4.  Both alpha-tubulin genes are transcriptionally active in Stylonychia lemnae.

Authors:  E Helftenbein; E Müller
Journal:  Curr Genet       Date:  1988-05       Impact factor: 3.886

5.  Nucleotide sequence of a macronuclear DNA molecule coding for alpha-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon.

Authors:  E Helftenbein
Journal:  Nucleic Acids Res       Date:  1985-01-25       Impact factor: 16.971

6.  Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

Authors:  M M Tondravi; M C Yao
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

7.  The organization of internal telomeric repeats in the polytene chromosomes of the hypotrichous ciliate Stylonychia lemnae.

Authors:  S Stoll; T Zirlik; C Maercker; H J Lipps
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

Review 8.  The DNA of ciliated protozoa.

Authors:  D M Prescott
Journal:  Microbiol Rev       Date:  1994-06

9.  Identification of an amplification promoting DNA sequence from the hypotrichous ciliate Stylonychia lemnae.

Authors:  M Wegner; E Helftenbein; F Müller; M Meinecke; S Müller; F Grummt
Journal:  Nucleic Acids Res       Date:  1989-11-11       Impact factor: 16.971

10.  Introduction of plasmid DNA into the trypanosomatid protozoan Crithidia fasciculata.

Authors:  D E Hughes; L Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205
  10 in total

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