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Literature DB >> 6296830

A DNA-mediated transformation system for Dictyostelium discoideum.

Abstract

We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10(-6) and 10(-4) of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.

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Year: 1982 PMID： 6296830 PMCID： PMC347338 DOI： 10.1073/pnas.79.23.7356

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

1. Characterization of the genome of the cellular slime mold Dictyostelium discoideum.

Authors: R A Firtel; J Bonner
Journal: J Mol Biol Date: 1972-05-28 Impact factor: 5.469

2. Sensitivity of Dictyostelium discoideum to nucleic acid analogues.

Authors: W F Loomis
Journal: Exp Cell Res Date: 1971-02 Impact factor: 3.905

3. Structural organization of the genome of Dictyostelium discoideum: analysis by EcoR1 restriction endonuclease.

Authors: R A Firtel; A Cockburn; G Frankel; V Hershfield
Journal: J Mol Biol Date: 1976-04-25 Impact factor: 5.469

4. Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genes.

Authors: K L Kindle; R A Firtel
Journal: Cell Date: 1978-11 Impact factor: 41.582

5. Analysis of the multigene family coding the developmentally regulated carbohydrate-binding protein discoidin-I in D. discoideum.

Authors: W Rowekamp; S Poole; R A Firtel
Journal: Cell Date: 1980-06 Impact factor: 41.582

6. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors: K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

7. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

8. Dictyostelium rDNA consists of non-chromosomal palindromic dimers containing 5S and 36S coding regions.

Authors: A F Cockburn; W C Taylor; R A Firtel
Journal: Chromosoma Date: 1978-12-21 Impact factor: 4.316

9. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.

Authors: A J Kingsman; L Clarke; R K Mortimer; J Carbon
Journal: Gene Date: 1979-10 Impact factor: 3.688

10. RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas.

Authors: S M Cocucci; M Sussman
Journal: J Cell Biol Date: 1970-05 Impact factor: 10.539

14 in total

1. Heterologous transformation of Agrocybe aegerita with a bacterial neomycin-resistance gene fused to a fungal promoter-like DNA sequence.

Authors: T Noël; P Simoneau; J Labarère
Journal: Theor Appl Genet Date: 1995-06 Impact factor: 5.699

2. Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

Authors: M M Tondravi; M C Yao
Journal: Proc Natl Acad Sci U S A Date: 1986-06 Impact factor: 11.205

3. Cloning and expression of a cDNA that comprises part of the gene coding for a spore coat protein of Dictyostelium discoideum.

Authors: B C Dowds; W F Loomis
Journal: Mol Cell Biol Date: 1984-11 Impact factor: 4.272

4. Isolation and characterization of DNA sequences from Triticum aestivum which function as origins of replication in Saccharomyces cerevisiae.

Authors: D André; J M Jacquemin; P Masson
Journal: Plant Cell Rep Date: 1983-08 Impact factor: 4.570