| Literature DB >> 16438968 |
Charles Sabin1, Edward P Mitchell, Martina Pokorná, Catherine Gautier, Jean-Pierre Utille, Michaela Wimmerová, Anne Imberty.
Abstract
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.Entities:
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Year: 2006 PMID： 16438968 DOI： 10.1016/j.febslet.2006.01.030
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124