Literature DB >> 16428426

Topologically fixed SecG is fully functional.

Eli O van der Sluis1, Erhard van der Vries, Greetje Berrelkamp, Nico Nouwen, Arnold J M Driessen.   

Abstract

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

Mesh：

Substances：

Year:  2006        PMID： 16428426      PMCID： PMC1347354          DOI： 10.1128/JB.188.3.1188-1190.2006

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  SecG is an auxiliary component of the protein export apparatus of Escherichia coli.

Authors:  A M Flower; L L Hines; P L Pfennig
Journal:  Mol Gen Genet       Date:  2000-02

2.  Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking.

Authors:  S Nagamori; K Nishiyama; H Tokuda
Journal:  J Biochem       Date:  2000-07       Impact factor: 3.387

3.  Covalently dimerized SecA is functional in protein translocation.

Authors:  Jeanine de Keyzer; Eli O van der Sluis; Robin E J Spelbrink; Niels Nijstad; Ben de Kruijff; Nico Nouwen; Chris van der Does; Arnold J M Driessen
Journal:  J Biol Chem       Date:  2005-08-22       Impact factor: 5.157

4.  Amino-terminal region of SecA is involved in the function of SecG for protein translocation into Escherichia coli membrane vesicles.

Authors:  H Mori; H Sugiyama; M Yamanaka; K Sato; M Tagaya; S Mizushima
Journal:  J Biochem       Date:  1998-07       Impact factor: 3.387

5.  Roles of SecG in ATP- and SecA-dependent protein translocation.

Authors:  G Matsumoto; H Mori; K Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-10       Impact factor: 11.205

6.  Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation.

Authors:  K Nishiyama; T Suzuki; H Tokuda
Journal:  Cell       Date:  1996-04-05       Impact factor: 41.582

7.  Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.

Authors:  F Duong; W Wickner
Journal:  EMBO J       Date:  1997-05-15       Impact factor: 11.598

8.  SecG function and phospholipid metabolism in Escherichia coli.

Authors:  A M Flower
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

9.  Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE.

Authors:  A Kaufmann; E H Manting; A K Veenendaal; A J Driessen; C van der Does
Journal:  Biochemistry       Date:  1999-07-13       Impact factor: 3.162

10.  Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY.

Authors:  Kurt S Cannon; Eran Or; William M Clemons; Yoko Shibata; Tom A Rapoport
Journal:  J Cell Biol       Date:  2005-04-25       Impact factor: 10.539
View more
  8 in total

1.  Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion.

Authors:  Kazuhiro Morita; Hajime Tokuda; Ken-ichi Nishiyama
Journal:  J Biol Chem       Date:  2011-11-10       Impact factor: 5.157

Review 2.  The bacterial Sec-translocase: structure and mechanism.

Authors:  Jelger A Lycklama A Nijeholt; Arnold J M Driessen
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2012-04-19       Impact factor: 6.237

3.  Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation.

Authors:  Michael Moser; Shushi Nagamori; Maria Huber; Hajime Tokuda; Ken-ichi Nishiyama
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-28       Impact factor: 11.205

4.  Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.

Authors:  Mark J J B Sibbald; Theresa Winter; Magdalena M van der Kooi-Pol; G Buist; E Tsompanidou; Tjibbe Bosma; Tina Schäfer; Knut Ohlsen; Michael Hecker; Haike Antelmann; Susanne Engelmann; Jan Maarten van Dijl
Journal:  J Bacteriol       Date:  2010-05-14       Impact factor: 3.490

5.  Mapping of the SecA·SecY and SecA·SecG interfaces by site-directed in vivo photocross-linking.

Authors:  Sanchaita Das; Donald B Oliver
Journal:  J Biol Chem       Date:  2011-02-11       Impact factor: 5.157

Review 6.  The Sec System: Protein Export in Escherichia coli.

Authors:  Jennine M Crane; Linda L Randall
Journal:  EcoSal Plus       Date:  2017-11

7.  Structure of a complex of the ATPase SecA and the protein-translocation channel.

Authors:  Jochen Zimmer; Yunsun Nam; Tom A Rapoport
Journal:  Nature       Date:  2008-10-16       Impact factor: 49.962

Review 8.  Mechanisms of integral membrane protein insertion and folding.

Authors:  Florian Cymer; Gunnar von Heijne; Stephen H White
Journal:  J Mol Biol       Date:  2014-09-30       Impact factor: 5.469
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.