Literature DB >> 16428419

Steady-state kinetic analysis of phosphotransacetylase from Methanosarcina thermophila.

Sarah H Lawrence1, James G Ferry.   

Abstract

Phosphotransacetylase (EC 2.3.1.8) catalyzes the reversible transfer of the acetyl group from acetyl phosphate to coenzyme A (CoA), forming acetyl-CoA and inorganic phosphate. A steady-state kinetic analysis of the phosphotransacetylase from Methanosarcina thermophila indicated that there is a ternary complex kinetic mechanism rather than a ping-pong kinetic mechanism. Additionally, inhibition patterns of products and a nonreactive substrate analog suggested that the substrates bind to the enzyme in a random order. Dynamic light scattering revealed that the enzyme is dimeric in solution.

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Year:  2006        PMID： 16428419      PMCID： PMC1347351          DOI： 10.1128/JB.188.3.1155-1158.2006

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila.

Authors:  Prabha P Iyer; Sarah H Lawrence; Kelvin B Luther; Kanagalaghatta R Rajashankar; Hemant P Yennawar; James G Ferry; Hermann Schindelin
Journal:  Structure       Date:  2004-04       Impact factor: 5.006

2.  Crystal structure of a phosphotransacetylase from Streptococcus pyogenes.

Authors:  Qian Steven Xu; Dong-Hae Shin; Ramona Pufan; Hisao Yokota; Rosalind Kim; Sung-Hou Kim
Journal:  Proteins       Date:  2004-05-01

Review 3.  Determining the chemical mechanisms of enzyme-catalyzed reactions by kinetic studies.

Authors:  W W Cleland
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1977

4.  Role of arginines in coenzyme A binding and catalysis by the phosphotransacetylase from Methanosarcina thermophila.

Authors:  P P Iyer; J G Ferry
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

5.  Kinetic studies with phosphotransacetylase. IV. Inhibition by products.

Authors:  S A Kyrtopoulos; D P Satchell
Journal:  Biochim Biophys Acta       Date:  1972-08-28

6.  Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila.

Authors:  M E Rasche; K S Smith; J G Ferry
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

7.  Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila.

Authors:  Sarah H Lawrence; Kelvin B Luther; Hermann Schindelin; James G Ferry
Journal:  J Bacteriol       Date:  2006-02       Impact factor: 3.490

8.  Evidence against an acyl-enzyme intermediate in the reaction catalyzed by clostridial phosphotransacetylase.

Authors:  J Henkin; R H Abeles
Journal:  Biochemistry       Date:  1976-08-10       Impact factor: 3.162

9.  Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila.

Authors:  M T Latimer; J G Ferry
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

10.  Expression, purification, crystallization and preliminary X-ray analysis of phosphotransacetylase from Methanosarcina thermophila.

Authors:  Prabha P Iyer; Sarah H Lawrence; Hemant P Yennawar; James G Ferry
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-07-23
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  10 in total

Review 1.  Structure and function of enzymes involved in the anaerobic degradation of L-threonine to propionate.

Authors:  Dhirendra K Simanshu; Sagar Chittori; H S Savithri; M R N Murthy
Journal:  J Biosci       Date:  2007-09       Impact factor: 1.826

2.  Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.

Authors:  Wenqiang Yang; Claudia Catalanotti; Sarah D'Adamo; Tyler M Wittkopp; Cheryl J Ingram-Smith; Luke Mackinder; Tarryn E Miller; Adam L Heuberger; Graham Peers; Kerry S Smith; Martin C Jonikas; Arthur R Grossman; Matthew C Posewitz
Journal:  Plant Cell       Date:  2014-11-07       Impact factor: 11.277

3.  Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila.

Authors:  Sarah H Lawrence; Kelvin B Luther; Hermann Schindelin; James G Ferry
Journal:  J Bacteriol       Date:  2006-02       Impact factor: 3.490

4.  Cloning, characterization and transcriptional analysis of two phosphate acetyltransferase isoforms from Azotobacter vinelandii.

Authors:  Maria Dimou; Anastasia Venieraki; Georgios Liakopoulos; Panagiotis Katinakis
Journal:  Mol Biol Rep       Date:  2010-11-21       Impact factor: 2.316

5.  The cytoplasmic cyclophilin from Azotobacter vinelandii interacts with phosphate acetyltransferase isoforms enhancing their in vitro activity.

Authors:  Maria Dimou; Anastasia Venieraki; Chrysoula Zografou; Panagiotis Katinakis
Journal:  Mol Biol Rep       Date:  2011-07-20       Impact factor: 2.316

6.  Biochemical and Kinetic Characterization of the Eukaryotic Phosphotransacetylase Class IIa Enzyme from Phytophthora ramorum.

Authors:  Tonya Taylor; Cheryl Ingram-Smith; Kerry S Smith
Journal:  Eukaryot Cell       Date:  2015-05-08

7.  Broad substrate specificity of phosphotransbutyrylase from Listeria monocytogenes: A potential participant in an alternative pathway for provision of acyl CoA precursors for fatty acid biosynthesis.

Authors:  Sirisha Sirobhushanam; Charitha Galva; Suranjana Sen; Brian J Wilkinson; Craig Gatto
Journal:  Biochim Biophys Acta       Date:  2016-06-15

8.  Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Authors:  Haiyan Liu; Zhongle Cheng; Wen Song; Wenyong Wu; Zheng Zhou
Journal:  PLoS One       Date:  2014-10-16       Impact factor: 3.240

9.  Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection.

Authors:  Yevgeniya I Shurubor; Marilena D'Aurelio; Joanne Clark-Matott; Elena P Isakova; Yulia I Deryabina; M Flint Beal; Arthur J L Cooper; Boris F Krasnikov
Journal:  Molecules       Date:  2017-08-23       Impact factor: 4.411

10.  Potential Role of Acetyl-CoA Synthetase (acs) and Malate Dehydrogenase (mae) in the Evolution of the Acetate Switch in Bacteria and Archaea.

Authors:  Elliott P Barnhart; Marcella A McClure; Kiki Johnson; Sean Cleveland; Kristopher A Hunt; Matthew W Fields
Journal:  Sci Rep       Date:  2015-08-03       Impact factor: 4.379
  10 in total

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