OBJECTIVE: The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model. MATERIALS AND METHODS: A second-generation, liposomal blood pool contrast agent encapsulating a high concentration of iodine (83-105 mg I/mL) was evaluated. A total of five mice weighing between 20 and 28 g were infused with equivalent volume doses (500 microL of contrast agent/25 g of mouse weight) and imaged with our micro-CT system for intervals of up to 240 min postinfusion. The animals were anesthetized, mechanically ventilated, and vital signs monitored allowing for simultaneous cardiac and respiratory gating of image acquisition. RESULTS: Initial enhancement of about 900 H in the aorta was obtained, which decreased to a plateau level of approximately 800 H after 2 hr. Excellent contrast discrimination was shown between the myocardium and cardiac blood pool (650-700 H). No significant nephrogram was identified, indicating the absence of renal clearance of the agent. CONCLUSION: The liposomal-based iodinated contrast agent shows long residence time in the blood pool, very high attenuation within submillimeter vessels, and no significant renal clearance rendering it an effective contrast agent for murine vascular imaging using a micro-CT scanner.
OBJECTIVE: The goal of this study was to determine if an iodinated, liposomal contrast agent could be used for high-resolution, micro-CT of low-contrast, small-size vessels in a murine model. MATERIALS AND METHODS: A second-generation, liposomal blood pool contrast agent encapsulating a high concentration of iodine (83-105 mg I/mL) was evaluated. A total of five mice weighing between 20 and 28 g were infused with equivalent volume doses (500 microL of contrast agent/25 g of mouse weight) and imaged with our micro-CT system for intervals of up to 240 min postinfusion. The animals were anesthetized, mechanically ventilated, and vital signs monitored allowing for simultaneous cardiac and respiratory gating of image acquisition. RESULTS: Initial enhancement of about 900 H in the aorta was obtained, which decreased to a plateau level of approximately 800 H after 2 hr. Excellent contrast discrimination was shown between the myocardium and cardiac blood pool (650-700 H). No significant nephrogram was identified, indicating the absence of renal clearance of the agent. CONCLUSION: The liposomal-based iodinated contrast agent shows long residence time in the blood pool, very high attenuation within submillimeter vessels, and no significant renal clearance rendering it an effective contrast agent for murine vascular imaging using a micro-CT scanner.
Authors: Ketan B Ghaghada; Cristian T Badea; Lohitash Karumbaiah; Nicole Fettig; Ravi V Bellamkonda; G A Johnson; Ananth Annapragada Journal: Acad Radiol Date: 2011-01 Impact factor: 3.173
Authors: Biana Godin; Jason H Sakamoto; Rita E Serda; Alessandro Grattoni; Ali Bouamrani; Mauro Ferrari Journal: Trends Pharmacol Sci Date: 2010-02-19 Impact factor: 14.819
Authors: Cristian T Badea; Laurence W Hedlund; Julie F Boslego Mackel; Lan Mao; Howard A Rockman; G Allan Johnson Journal: Mol Imaging Date: 2007 Jul-Aug Impact factor: 4.488
Authors: Chang-Lung Lee; Hooney Min; Nicholas Befera; Darin Clark; Yi Qi; Shiva Das; G Allan Johnson; Cristian T Badea; David G Kirsch Journal: Int J Radiat Oncol Biol Phys Date: 2014-03-01 Impact factor: 7.038
Authors: Jeffrey R Ashton; Nicholas Befera; Darin Clark; Yi Qi; Lan Mao; Howard A Rockman; G Allan Johnson; Cristian T Badea Journal: Contrast Media Mol Imaging Date: 2014 Mar-Apr Impact factor: 3.161