BACKGROUND AND AIMS: Gastric mucosa associated lymphoid tissue lymphoma is a well defined B cell lymphoma yet often impossible to distinguish from severe chronic gastritis on morphological grounds alone. Therefore, it was suggested to use the clonality of the immunoglobulin (Ig) heavy chain (H) genes, as detected by polymerase chain reaction (PCR), as a decisive criterion. However, there is controversy as to whether B cell clonality also exists in chronic gastritis, hence rendering this approach futile at present. METHODS: An expert panel re-examined the histology and immunohistochemistry of a total of 97 cases of gastric biopsies, including clearcut marginal zone lymphoma, chronic gastritis, and ambiguous cases, applying the Wotherspoon criteria on the basis of haematoxylin-eosin and CD20 immunostainings. In addition, a new and advanced PCR system for detection of clonal IgH gene rearrangements was independently applied in two institutions in each case. RESULTS: The overall IgH clonality assessments of both institutions were in total agreement. Overt lymphoma (Wotherspoon score 5) was clonal in 24/26 cases. Chronic gastritis (Wotherspoon scores 1 and 2) was not clonal in 52/53 cases; the clonal case being Wotherspoon score 2. Of 18 cases with ambiguous histology (Wotherspoon scores 3 and 4) four were clonal. CONCLUSIONS: Using advanced PCR technology, clonal gastritis is extremely rare, if it exists at all. Thus B cell clonality in Wotherspoon 3 and 4 cases is regarded as suitable for definitively diagnosing gastric marginal zone lymphoma.
BACKGROUND AND AIMS: Gastric mucosa associated lymphoid tissue lymphoma is a well defined B cell lymphoma yet often impossible to distinguish from severe chronic gastritis on morphological grounds alone. Therefore, it was suggested to use the clonality of the immunoglobulin (Ig) heavy chain (H) genes, as detected by polymerase chain reaction (PCR), as a decisive criterion. However, there is controversy as to whether B cell clonality also exists in chronic gastritis, hence rendering this approach futile at present. METHODS: An expert panel re-examined the histology and immunohistochemistry of a total of 97 cases of gastric biopsies, including clearcut marginal zone lymphoma, chronic gastritis, and ambiguous cases, applying the Wotherspoon criteria on the basis of haematoxylin-eosin and CD20 immunostainings. In addition, a new and advanced PCR system for detection of clonal IgH gene rearrangements was independently applied in two institutions in each case. RESULTS: The overall IgH clonality assessments of both institutions were in total agreement. Overt lymphoma (Wotherspoon score 5) was clonal in 24/26 cases. Chronic gastritis (Wotherspoon scores 1 and 2) was not clonal in 52/53 cases; the clonal case being Wotherspoon score 2. Of 18 cases with ambiguous histology (Wotherspoon scores 3 and 4) four were clonal. CONCLUSIONS: Using advanced PCR technology, clonal gastritis is extremely rare, if it exists at all. Thus B cell clonality in Wotherspoon 3 and 4 cases is regarded as suitable for definitively diagnosing gastric marginal zone lymphoma.
Authors: T F Barth; M Bentz; F Leithäuser; S Stilgenbauer; R Siebert; M Schlotter; R F Schlenk; H Döhner; P Möller Journal: Genes Chromosomes Cancer Date: 2001-08 Impact factor: 5.006
Authors: Petr Starostik; Jochen Patzner; Axel Greiner; Stephan Schwarz; Jörg Kalla; German Ott; Hans Konrad Müller-Hermelink Journal: Blood Date: 2002-01-01 Impact factor: 22.113
Authors: A D Cabras; S Candidus; F Fend; M Kremer; S Schulz; C Bordi; G Weirich; H Höfler; M Werner Journal: Lab Invest Date: 2001-07 Impact factor: 5.662
Authors: S B Cogliatti; U Schmid; U Schumacher; F Eckert; M L Hansmann; J Hedderich; H Takahashi; K Lennert Journal: Gastroenterology Date: 1991-11 Impact factor: 22.682
Authors: A Savio; G Franzin; A C Wotherspoon; G Zamboni; R Negrini; F Buffoli; T C Diss; L Pan; P G Isaacson Journal: Blood Date: 1996-02-15 Impact factor: 22.113
Authors: Keyur P Patel; Qiulu Pan; Yanhua Wang; Robert W Maitta; Juan Du; Xiaonan Xue; Juan Lin; Howard Ratech Journal: J Mol Diagn Date: 2010-03 Impact factor: 5.568
Authors: Christoph Thorns; Johannes Kuba; Veronica Bernard; Andrea Senft; Silke Szymczak; Alfred C Feller; Heinz-Wolfram Bernd Journal: Virchows Arch Date: 2012-03-07 Impact factor: 4.064
Authors: Andrea Morgner; Renate Schmelz; Christian Thiede; Manfred Stolte; Stephan Miehlke Journal: World J Gastroenterol Date: 2007-07-14 Impact factor: 5.742