| Literature DB >> 16423070 |
Philippe Perpète1, Olivier Duthoit, Simon De Maeyer, Louise Imray, Andrew I Lawton, Konstantinos E Stavropoulos, Virginia W Gitonga, Michael J E Hewlins, J Richard Dickinson.
Abstract
The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields methional, followed by reduction. The decarboxylation is effected specifically by Ydr380wp. Methanethiol is formed from both methionine and alpha-keto-gamma-(methylthio)butyrate by a demethiolase activity. In all except one strain examined, demethiolase was induced by the presence of methionine in the growth medium. This pathway results in the production of alpha-ketobutyrate, a carbon skeleton, which can be re-utilized. Hence, methionine catabolism is more complex and economical than the other amino acid catabolic pathways in yeast, which use the Ehrlich pathway and result solely in the formation of a fusel alcohol.Entities:
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Year: 2006 PMID： 16423070 DOI： 10.1111/j.1567-1356.2005.00005.x
Source DB: PubMed Journal: FEMS Yeast Res ISSN： 1567-1356 Impact factor: 2.796