Lian-fang Zhou1, Yong-ping Zhu. 1. Department of Toxicology, School of Medicine, Zhejiang University, Hangzhou 310006, China. zhoulianfang@163.com
Abstract
OBJECTIVE: To investigate the changes in CREB (cAMP response element binding protein) in hippocampus, PFC (prefrontal cortex) and NAc (nucleus accumbens) during three phases of morphine induced CPP (conditioned place preference) in rats, and to elucidate the role of CREB during the progress of conditioned place preference. METHODS: Morphine induced CPP acquisition, extinction and drug primed reinstatement model was established, and CREB expression in each brain area was measured by Western Blot methods. RESULTS: Eight alternating injections of morphine (10 mg/kg) induced CPP, and 8 d saline extinction training that extinguished CPP. CPP was reinstated following a priming injection of morphine (2.5 mg/kg). During the phases of CPP acquisition and reinstatement, the level of CREB expression was significantly changed in different brain areas. CONCLUSION: It was proved that CPP model can be used as an effective tool to investigate the mechanisms underlying drug-induced reinstatement of drug seeking after extinction, and that morphine induced CPP and drug primed reinstatement may involve activation of the transcription factor CREB in several brain areas, suggesting that the CREB and its target gene regulation pathway may mediate the basic mechanism underlying opioid dependence and its drug seeking behavior.
OBJECTIVE: To investigate the changes in CREB (cAMP response element binding protein) in hippocampus, PFC (prefrontal cortex) and NAc (nucleus accumbens) during three phases of morphine induced CPP (conditioned place preference) in rats, and to elucidate the role of CREB during the progress of conditioned place preference. METHODS:Morphine induced CPP acquisition, extinction and drug primed reinstatement model was established, and CREB expression in each brain area was measured by Western Blot methods. RESULTS: Eight alternating injections of morphine (10 mg/kg) induced CPP, and 8 d saline extinction training that extinguished CPP. CPP was reinstated following a priming injection of morphine (2.5 mg/kg). During the phases of CPP acquisition and reinstatement, the level of CREB expression was significantly changed in different brain areas. CONCLUSION: It was proved that CPP model can be used as an effective tool to investigate the mechanisms underlying drug-induced reinstatement of drug seeking after extinction, and that morphine induced CPP and drug primed reinstatement may involve activation of the transcription factor CREB in several brain areas, suggesting that the CREB and its target gene regulation pathway may mediate the basic mechanism underlying opioid dependence and its drug seeking behavior.
Authors: Michel Barrot; Jocelien D A Olivier; Linda I Perrotti; Ralph J DiLeone; Olivier Berton; Amelia J Eisch; Soren Impey; Daniel R Storm; Rachael L Neve; Jerry C Yin; Venetia Zachariou; Eric J Nestler Journal: Proc Natl Acad Sci U S A Date: 2002-08-06 Impact factor: 11.205
Authors: Cassandra O Subiah; Musa V Mabandla; Alisa Phulukdaree; Anil A Chuturgoon; Willie M U Daniels Journal: Metab Brain Dis Date: 2012-03-25 Impact factor: 3.584
Authors: Patrick Recinto; Anjali Rose H Samant; Gustavo Chavez; Airee Kim; Clara J Yuan; Matthew Soleiman; Yanabel Grant; Scott Edwards; Sunmee Wee; George F Koob; Olivier George; Chitra D Mandyam Journal: Neuropsychopharmacology Date: 2011-12-28 Impact factor: 7.853