| Literature DB >> 16415889 |
Renae L Malek1, Hong-ying Wang, Anne E Kwitek, Andrew S Greene, Nirmal Bhagabati, Gretta Borchardt, Lisa Cahill, Tracey Currier, Bryan Frank, Xianping Fu, Michael Hasinoff, Eleanor Howe, Noah Letwin, Truong V Luu, Alexander Saeed, Hedieh Sajadi, Steven L Salzberg, Razvan Sultana, Mathangi Thiagarajan, Jennifer Tsai, Kathleen Veratti, Joseph White, John Quackenbush, Howard J Jacob, Norman H Lee.
Abstract
Cardiovascular disorders are influenced by genetic and environmental factors. The TIGR rodent expression web-based resource (TREX) contains over 2,200 microarray hybridizations, involving over 800 animals from 18 different rat strains. These strains comprise genetically diverse parental animals and a panel of chromosomal substitution strains derived by introgressing individual chromosomes from normotensive Brown Norway (BN/NHsdMcwi) rats into the background of Dahl salt sensitive (SS/JrHsdMcwi) rats. The profiles document gene-expression changes in both genders, four tissues (heart, lung, liver, kidney) and two environmental conditions (normoxia, hypoxia). This translates into almost 400 high-quality direct comparisons (not including replicates) and over 100,000 pairwise comparisons. As each individual chromosomal substitution strain represents on average less than a 5% change from the parental genome, consomic strains provide a useful mechanism to dissect complex traits and identify causative genes. We performed a variety of data-mining manipulations on the profiles and used complementary physiological data from the PhysGen resource to demonstrate how TREX can be used by the cardiovascular community for hypothesis generation.Entities:
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Year: 2006 PMID： 16415889 DOI： 10.1038/ng1693
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330