Literature DB >> 16408950

Genotoxicity screening using biocatalyst/DNA films and capillary LC-MS/MS.

Maricar Tarun, Besnik Bajrami, James F Rusling.   

Abstract

Detection of DNA adducts can serve as a basis for genotoxicty screening of new chemicals and drugs. We report here a simple, sensitive procedure for this purpose using films containing DNA and a biocatalyst to mimic the metabolic action of human liver cytochrome P450s. DNA adducts formed from an in-situ-generated toxic metabolite (styrene oxide) were detected at subpicomole levels after neutral thermal hydrolysis of the DNA films and analysis with capillary liquid chromatography with on-line column preconcentration and MS/MS detection. An on-line column switching system allowed for increased sample loading volume and analyte preconcentration. This approach provides an estimate of the relative rate of DNA damage.

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Year:  2006        PMID： 16408950     DOI： 10.1021/ac0517996

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  10 in total

Review 1.  Biochemical applications of ultrathin films of enzymes, polyions and DNA.

Authors:  James F Rusling; Eli G Hvastkovs; Dominic O Hull; John B Schenkman
Journal:  Chem Commun (Camb)       Date:  2007-08-30       Impact factor: 6.222

2.  Electrochemiluminescent/voltammetric toxicity screening sensor using enzyme-generated DNA damage.

Authors:  Minjeong So; Eli G Hvastkovs; John B Schenkman; James F Rusling
Journal:  Biosens Bioelectron       Date:  2007-07-28       Impact factor: 10.618

3.  Electrochemiluminescent arrays for cytochrome P450-activated genotoxicity screening. DNA damage from benzo[a]pyrene metabolites.

Authors:  Eli G Hvastkovs; Minjeong So; Sadagopan Krishnan; Besnik Bajrami; Maricar Tarun; Ingela Jansson; John B Schenkman; James F Rusling
Journal:  Anal Chem       Date:  2007-01-30       Impact factor: 6.986

Review 4.  Metabolic toxicity screening using electrochemiluminescence arrays coupled with enzyme-DNA biocolloid reactors and liquid chromatography-mass spectrometry.

Authors:  Eli G Hvastkovs; John B Schenkman; James F Rusling
Journal:  Annu Rev Anal Chem (Palo Alto Calif)       Date:  2012-04-05       Impact factor: 10.745

5.  Electrochemical DNA Sensor Based on Carbon Black-Poly(Methylene Blue)-Poly(Neutral Red) Composite.

Authors:  Dominica Kappo; Dmitry Shurpik; Pavel Padnya; Ivan Stoikov; Alexey Rogov; Gennady Evtugyn
Journal:  Biosensors (Basel)       Date:  2022-05-12

6.  Rapid LC-MS drug metabolite profiling using microsomal enzyme bioreactors in a parallel processing format.

Authors:  Besnik Bajrami; Linlin Zhao; John B Schenkman; James F Rusling
Journal:  Anal Chem       Date:  2009-12-15       Impact factor: 6.986

7.  Microsome biocolloids for rapid drug metabolism and inhibition assessment by LC-MS.

Authors:  Besnik Bajrami; Sadagopan Krishnan; James F Rusling
Journal:  Drug Metab Lett       Date:  2008-08

8.  Characterizing metabolic inhibition using electrochemical enzyme/DNA biosensors.

Authors:  Dominic O Hull; Besnik Bajrami; Ingela Jansson; John B Schenkman; James F Rusling
Journal:  Anal Chem       Date:  2009-01-15       Impact factor: 6.986

9.  Accurate DNA fragment sizing by capillary electrophoresis with laser-induced fluorescence array for detection of sequence specificity of DNA damage.

Authors:  Erwin V Fundador; Dharamainder Choudhary; John B Schenkman; James F Rusling
Journal:  Anal Chem       Date:  2008-02-12       Impact factor: 6.986

10.  Elucidating Organ-Specific Metabolic Toxicity Chemistry from Electrochemiluminescent Enzyme/DNA Arrays and Bioreactor Bead-LC-MS/MS.

Authors:  Dhanuka P Wasalathanthri; Dandan Li; Donghui Song; Zhifang Zheng; Dharamainder Choudhary; Ingela Jansson; Xiuling Lu; John B Schenkman; James F Rusling
Journal:  Chem Sci       Date:  2015       Impact factor: 9.825
  10 in total

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