Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Anucleate cell blue assay: a useful tool for identifying novel type II topoisomerase inhibitors.

Literature DB >> 16377708

Anucleate cell blue assay: a useful tool for identifying novel type II topoisomerase inhibitors.

Yoshihiro Oyamada¹, Hideaki Ito, Mika Fujimoto-Nakamura, Akihiko Tanitame, Noritaka Iwai, Kazuo Nagai, Jun-Ichi Yamagishi, Masaaki Wachi.

Abstract

About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.

Entities: Chemical Species

Mesh：

Substances：

Year: 2006 PMID： 16377708 PMCID： PMC1346804 DOI： 10.1128/AAC.50.1.348-350.2006

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

13 in total

1. Analysis of topoisomerase function in bacterial replication fork movement: use of DNA microarrays.

Authors: A B Khodursky; B J Peter; M B Schmid; J DeRisi; D Botstein; P O Brown; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 2000-08-15 Impact factor: 11.205

2. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells.

Authors: S Hiraga; H Niki; T Ogura; C Ichinose; H Mori; B Ezaki; A Jaffé
Journal: J Bacteriol Date: 1989-03 Impact factor: 3.490

3. DNA strand cleavage is required for replication fork arrest by a frozen topoisomerase-quinolone-DNA ternary complex.

Authors: H Hiasa; D O Yousef; K J Marians
Journal: J Biol Chem Date: 1996-10-18 Impact factor: 5.157

4. Inhibition of deoxyribonucleic acid gyrase: effects on nucleic acid synthesis and cell division in Escherichia coli K-12.

Authors: N F Fairweather; E Orr; I B Holland
Journal: J Bacteriol Date: 1980-04 Impact factor: 3.490

5. Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA.

Authors: M Wachi; N Iwai; A Kunihisa; K Nagai
Journal: Biochimie Date: 1999 Aug-Sep Impact factor: 4.079

6. Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.

Authors: K Sato; Y Inoue; T Fujii; H Aoyama; M Inoue; S Mitsuhashi
Journal: Antimicrob Agents Chemother Date: 1986-11 Impact factor: 5.191

7. Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus.

Authors: J Yamagishi; T Kojima; Y Oyamada; K Fujimoto; H Hattori; S Nakamura; M Inoue
Journal: Antimicrob Agents Chemother Date: 1996-05 Impact factor: 5.191

8. Accumulation of mutations in both gyrB and parE genes is associated with high-level resistance to novobiocin in Staphylococcus aureus.

Authors: Mika Fujimoto-Nakamura; Hideaki Ito; Yoshihiro Oyamada; Takeshi Nishino; Jun-Ichi Yamagishi
Journal: Antimicrob Agents Chemother Date: 2005-09 Impact factor: 5.191

9. Novel S-benzylisothiourea compound that induces spherical cells in Escherichia coli probably by acting on a rod-shape-determining protein(s) other than penicillin-binding protein 2.

Authors: Noritaka Iwai; Kazuo Nagai; Masaaki Wachi
Journal: Biosci Biotechnol Biochem Date: 2002-12 Impact factor: 2.043