| Literature DB >> 16376070 |
Abstract
Stable-isotope probing (SIP) of environmental genomic DNA allows populations in a natural microbial community to be identified through the incorporation of (13)C- or (15)N-labeled substrates into biomass. The isotopically labeled DNA is retrieved by density gradient centrifugation, and active microorganisms identified by molecular analysis of the DNA. Recent advances have set the stage for DNA-based SIP with higher sensitivities, shorter incubation times, and more relevant substrate concentrations than used previously. The first demonstration of a DNA-SIP-based metagenome analysis has paved the way for hypothesis-driven studies in environmental genomics.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16376070 DOI： 10.1016/j.copbio.2005.12.003
Source DB: PubMed Journal: Curr Opin Biotechnol ISSN： 0958-1669 Impact factor: 9.740