| Literature DB >> 16369549 |
Wigard P Kloosterman1, Erno Wienholds, Ewart de Bruijn, Sakari Kauppinen, Ronald H A Plasterk.
Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.Entities:
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Year: 2006 PMID： 16369549 DOI： 10.1038/nmeth843
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547