Literature DB >> 16348612

Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.

K P Putzer1, L A Buchholz, M E Lidstrom, C C Remsen.   

Abstract

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

Entities:  

Year:  1991        PMID： 16348612      PMCID： PMC184030          DOI： 10.1128/aem.57.12.3656-3659.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  Separation of Syntrophomonas wolfei from Methanospirillum hungatii in Syntrophic Cocultures by Using Percoll Gradients.

Authors:  P S Beaty; N Q Wofford; M J McInerney
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

2.  Specific binding of rat liver plasma membranes by rat liver cells.

Authors:  B Obrink; B Wärmegård; H Pertoft
Journal:  Biochem Biophys Res Commun       Date:  1977-07-25       Impact factor: 3.575

3.  Harvest of planktonic marine algae by centrifugation into gradients of silica in the CF-6 continuous-flow zonal rotor.

Authors:  C A Price; L R Mendiola-Morgenthaler; M Goldstein; E N Breden; R R Guillard
Journal:  Biol Bull       Date:  1974-08       Impact factor: 1.818

4.  Enrichment, isolation and some properties of methane-utilizing bacteria.

Authors:  R Whittenbury; K C Phillips; J F Wilkinson
Journal:  J Gen Microbiol       Date:  1970-05

5.  Isolation and characterization of marine methanotrophs.

Authors:  M E Lidstrom
Journal:  Antonie Van Leeuwenhoek       Date:  1988       Impact factor: 2.271

6.  Discontinuous density gradient separation of human mononuclear leucocytes using Percoll as gradient medium.

Authors:  A J Ulmer; H D Flad
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

Review 7.  A survey of buoyant density of microorganisms in pure cultures and natural samples.

Authors:  R Guerrero; C Pedrós-Alió; T M Schmidt; J Mas
Journal:  Microbiologia       Date:  1985-09

8.  A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continuous density gradients of Percoll.

Authors:  A W Segal; A Fortunato; T Herd
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

9.  Use of oligodeoxynucleotide signature probes for identification of physiological groups of methylotrophic bacteria.

Authors:  H C Tsien; B J Bratina; K Tsuji; R S Hanson
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792
  9 in total
  5 in total

1.  Density-dependent sorting of physiologically different cells of Vibrio parahaemolyticus.

Authors:  Tomohiko Nishino; Binaya B Nayak; Kazuhiro Kogure
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

2.  Localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling.

Authors:  T A Fassel; L A Buchholz; M L Collins; C C Remsen
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

3.  Applications of a colorimetric plate assay for soluble methane monooxygenase activity.

Authors:  D W Graham; D G Korich; R P LeBlanc; N A Sinclair; R G Arnold
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

4.  Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contaminated soil.

Authors:  J K Struthers; K Jayachandran; T B Moorman
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

5.  Factors affecting competition between type I and type II methanotrophs in two-organism, continuous-flow reactors.

Authors:  D W Graham; J A Chaudhary; R S Hanson; R G Arnold
Journal:  Microb Ecol       Date:  1993-01       Impact factor: 4.552
  5 in total

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