Literature DB >> 16347346

Separation of Syntrophomonas wolfei from Methanospirillum hungatii in Syntrophic Cocultures by Using Percoll Gradients.

P S Beaty1, N Q Wofford, M J McInerney.   

Abstract

Percoll gradient centrifugation effectively separated Syntrophomonas wolfei cells from Methanospirillum hungatii cells, resulting in a 70- to 80-fold enrichment of S. wolfei cells relative to M. hungatii cells. The separated S. wolfei cells were viable. Gram quantities of cellular protein which was enzymatically active and had low levels of contamination by the methanogenic cofactor, factor(420), were obtained.

Entities:  

Year:  1987        PMID： 16347346      PMCID： PMC203831          DOI： 10.1128/aem.53.5.1183-1185.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO(2) to Methanogenesis in Cattle Waste at 40 and 60 degrees C.

Authors:  R I Mackie; M P Bryant
Journal:  Appl Environ Microbiol       Date:  1981-06       Impact factor: 4.792

3.  Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium.

Authors:  M J McInerney; M P Bryant; R B Hespell; J W Costerton
Journal:  Appl Environ Microbiol       Date:  1981-04       Impact factor: 4.792

4.  Isolation of a Butyrate-Utilizing Bacterium in Coculture with Methanobacterium thermoautotrophicum from a Thermophilic Digester.

Authors:  J M Henson; P H Smith
Journal:  Appl Environ Microbiol       Date:  1985-06       Impact factor: 4.792

5.  Propionate-Degrading Bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from Methanogenic Ecosystems.

Authors:  D R Boone; M P Bryant
Journal:  Appl Environ Microbiol       Date:  1980-09       Impact factor: 4.792

6.  Isolation and properties of a fluorescent compound, factor 420 , from Methanobacterium strain M.o.H.

Authors:  P Cheeseman; A Toms-Wood; R S Wolfe
Journal:  J Bacteriol       Date:  1972-10       Impact factor: 3.490

7.  New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere.

Authors:  W E Balch; R S Wolfe
Journal:  Appl Environ Microbiol       Date:  1976-12       Impact factor: 4.792

8.  Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge.

Authors:  H F Kaspar; K Wuhrmann
Journal:  Appl Environ Microbiol       Date:  1978-07       Impact factor: 4.792

9.  Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species.

Authors:  B R Genthner; C L Davis; M P Bryant
Journal:  Appl Environ Microbiol       Date:  1981-07       Impact factor: 4.792

10.  Preparation of cell-free extracts and the enzymes involved in fatty acid metabolism in Syntrophomonas wolfei.

Authors:  N Q Wofford; P S Beaty; M J McInerney
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490
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  10 in total

1.  Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.

Authors:  K P Putzer; L A Buchholz; M E Lidstrom; C C Remsen
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

Review 2.  Energetics of syntrophic cooperation in methanogenic degradation.

Authors:  B Schink
Journal:  Microbiol Mol Biol Rev       Date:  1997-06       Impact factor: 11.056

Review 3.  Innovations to culturing the uncultured microbial majority.

Authors:  William H Lewis; Guillaume Tahon; Patricia Geesink; Diana Z Sousa; Thijs J G Ettema
Journal:  Nat Rev Microbiol       Date:  2020-10-22       Impact factor: 60.633

4.  Differential expression of methanogenesis genes of Methanothermobacter thermoautotrophicus (formerly Methanobacterium thermoautotrophicum) in pure culture and in cocultures with fatty acid-oxidizing syntrophs.

Authors:  Hong-Wei Luo; Hui Zhang; Toshihiko Suzuki; Satoshi Hattori; Yoichi Kamagata
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

5.  Biochemical evidence for formate transfer in syntrophic propionate-oxidizing cocultures of Syntrophobacter fumaroxidans and Methanospirillum hungatei.

Authors:  Frank A M de Bok; Maurice L G C Luijten; Alfons J M Stams
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

6.  Involvement of NADH:acceptor oxidoreductase and butyryl coenzyme A dehydrogenase in reversed electron transport during syntrophic butyrate oxidation by Syntrophomonas wolfei.

Authors:  Nicolai Müller; David Schleheck; Bernhard Schink
Journal:  J Bacteriol       Date:  2009-07-31       Impact factor: 3.490

7.  Effect of propionate toxicity on methanogen-enriched sludge, Methanobrevibacter smithii, and Methanospirillum hungatii at different pH values.

Authors:  M S Barredo; L M Evison
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

8.  Membrane Complexes of Syntrophomonas wolfei Involved in Syntrophic Butyrate Degradation and Hydrogen Formation.

Authors:  Bryan R Crable; Jessica R Sieber; Xinwei Mao; Lisa Alvarez-Cohen; Robert Gunsalus; Rachel R Ogorzalek Loo; Hong Nguyen; Michael J McInerney
Journal:  Front Microbiol       Date:  2016-11-09       Impact factor: 5.640

9.  A pyrosequencing-based metagenomic study of methane-producing microbial community in solid-state biogas reactor.

Authors:  An Li; Ya'nan Chu; Xumin Wang; Lufeng Ren; Jun Yu; Xiaoling Liu; Jianbin Yan; Lei Zhang; Shuangxiu Wu; Shizhong Li
Journal:  Biotechnol Biofuels       Date:  2013-01-15       Impact factor: 6.040

10.  A proteomic view at the biochemistry of syntrophic butyrate oxidation in Syntrophomonas wolfei.

Authors:  Alexander Schmidt; Nicolai Müller; Bernhard Schink; David Schleheck
Journal:  PLoS One       Date:  2013-02-26       Impact factor: 3.240
  10 in total

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