Literature DB >> 16348200

Simple, sensitive zymogram technique for detection of xylanase activity in polyacrylamide gels.

J C Royer1, J P Nakas.   

Abstract

A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.

Entities:  

Year:  1990        PMID： 16348200      PMCID： PMC184463          DOI： 10.1128/aem.56.6.1516-1517.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors:  R A REISFELD; U J LEWIS; D E WILLIAMS
Journal:  Nature       Date:  1962-07-21       Impact factor: 49.962

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Purification and Characterization of an Endo-(1,3)-beta-d-Glucanase from Trichoderma longibrachiatum.

Authors:  B Tangarone; J C Royer; J P Nakas
Journal:  Appl Environ Microbiol       Date:  1989-01       Impact factor: 4.792

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  The basis for colored silver-protein complex formation in stained polyacrylamide gels.

Authors:  B L Nielsen; L R Brown
Journal:  Anal Biochem       Date:  1984-09       Impact factor: 3.365

6.  Sensitive detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in gels.

Authors:  P Biely; O Markovic; D Mislovicová
Journal:  Anal Biochem       Date:  1985-01       Impact factor: 3.365
  6 in total
  9 in total

1.  Interrelationship of Xylanase Induction and Cellulase Induction of Trichoderma longibrachiatum.

Authors:  John C Royer; J P Nakas
Journal:  Appl Environ Microbiol       Date:  1990-08       Impact factor: 4.792

2.  Xylanase Activity of Phanerochaete chrysosporium.

Authors:  M S Dobozi; G Szakács; C V Bruschi
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

3.  alpha-Glucuronidase and Other Hemicellulase Activities of Fibrobacter succinogenes S85 Grown on Crystalline Cellulose or Ball-Milled Barley Straw.

Authors:  D C Smith; C W Forsberg
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

4.  Characterization, cloning and functional expression of novel xylanase from Thermomyces lanuginosus SS-8 isolated from self-heating plant wreckage material.

Authors:  Smriti Shrivastava; Pratyoosh Shukla; Putchen Dakshinamoorthy Deepalakshmi; Kunal Mukhopadhyay
Journal:  World J Microbiol Biotechnol       Date:  2013-06-23       Impact factor: 3.312

5.  Purification and properties of ArfI, an alpha-L-arabinofuranosidase from Cytophaga xylanolytica.

Authors:  M J Renner; J A Breznak
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

6.  Purification and characterization of a new xylanase (APX-II) from the fungus Aureobasidium pullulans Y-2311-1.

Authors:  X L Li; Z Q Zhang; J F Dean; K E Eriksson; L G Ljungdahl
Journal:  Appl Environ Microbiol       Date:  1993-10       Impact factor: 4.792

7.  Secretome analysis of the thermophilic xylanase hyper-producer Thermomyces lanuginosus SSBP cultivated on corn cobs.

Authors:  A M Winger; J L Heazlewood; L J G Chan; C J Petzold; K Permaul; S Singh
Journal:  J Ind Microbiol Biotechnol       Date:  2014-09-16       Impact factor: 3.346

8.  Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA.

Authors:  María Teresa Alvarez-Zúñiga; Alejandro Santiago-Hernández; Johan Rodríguez-Mendoza; Jorge E Campos; Patricia Pavón-Orozco; Sergio Trejo-Estrada; María Eugenia Hidalgo-Lara
Journal:  AMB Express       Date:  2017-11-02       Impact factor: 3.298

9.  Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme.

Authors:  Jun He; Bing Yu; Keying Zhang; Xuemei Ding; Daiwen Chen
Journal:  BMC Biotechnol       Date:  2009-06-16       Impact factor: 2.563
  9 in total

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