Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA.

Literature DB >> 16347975

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA.

Abstract

Cloning of cDNA obtained from 16S rRNA (16S rcDNA) selectively retrieves species-specific sequence information useful for analyzing the composition and structure of natural microbial communities. With this technique we obtained recombinant 16S rcDNA libraries from Escherichia coli and from a model hot-spring cyanobacterial-mat community. The recombinant plasmids contained exclusively 16S rRNA-derived inserts. This selective approach is independent of biasing culture techniques and eliminates the laborious screening required to locate 16S rRNA gene-bearing recombinants in genomic DNA libraries obtained from natural communities.

Entities: Chemical Species

Year: 1989 PMID： 16347975 PMCID： PMC202956 DOI： 10.1128/aem.55.7.1818-1822.1989

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

17 in total

1. A phylogenetic definition of the major eubacterial taxa.

Authors: C R Woese; E Stackebrandt; T J Macke; G E Fox
Journal: Syst Appl Microbiol Date: 1985 Impact factor: 4.022

2. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors: William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal: Appl Environ Microbiol Date: 1988-03 Impact factor: 4.792

Review 3. Bacterial evolution.

Authors: C R Woese
Journal: Microbiol Rev Date: 1987-06

4. Structure, growth, and decomposition of laminated algal-bacterial mats in alkaline hot springs.

Authors: W N Doemel; T D Brock
Journal: Appl Environ Microbiol Date: 1977-10 Impact factor: 4.792

5. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors: S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal: J Bacteriol Date: 1988-02 Impact factor: 3.490

6. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.

Authors: E F DeLong; G S Wickham; N R Pace
Journal: Science Date: 1989-03-10 Impact factor: 47.728

7. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors: J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

8. Mechanism of action of Moloney murine leukemia virus RNA-directed DNA polymerase associated RNase H (RNase H I).

Authors: G F Gerard
Journal: Biochemistry Date: 1981-01-20 Impact factor: 3.162

9. Conserved 5S rRNA complement to tRNA is not required for protein synthesis.

Authors: B Pace; E A Matthews; K D Johnson; C R Cantor; N R Pace
Journal: Proc Natl Acad Sci U S A Date: 1982-01 Impact factor: 11.205

10. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

Authors: D A Stahl; B Flesher; H R Mansfield; L Montgomery
Journal: Appl Environ Microbiol Date: 1988-05 Impact factor: 4.792

25 in total

1. Soil type is the primary determinant of the composition of the total and active bacterial communities in arable soils.

Authors: Martina S Girvan; Juliet Bullimore; Jules N Pretty; A Mark Osborn; Andrew S Ball
Journal: Appl Environ Microbiol Date: 2003-03 Impact factor: 4.792

2. Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing.

Authors: T M Schmidt; E F DeLong; N R Pace
Journal: J Bacteriol Date: 1991-07 Impact factor: 3.490

3. Comparison of 16S rRNA and 16S rDNA T-RFLP approaches to study bacterial communities in soil microcosms treated with chromate as perturbing agent.

Authors: Alessio Mengoni; Enrico Tatti; Francesca Decorosi; Carlo Viti; Marco Bazzicalupo; Luciana Giovannetti
Journal: Microb Ecol Date: 2005-11-03 Impact factor: 4.552

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA.

1. A phylogenetic definition of the major eubacterial taxa.

2. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Review 3. Bacterial evolution.

4. Structure, growth, and decomposition of laminated algal-bacterial mats in alkaline hot springs.

5. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

6. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.

7. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

8. Mechanism of action of Moloney murine leukemia virus RNA-directed DNA polymerase associated RNase H (RNase H I).

9. Conserved 5S rRNA complement to tRNA is not required for protein synthesis.

10. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

1. Soil type is the primary determinant of the composition of the total and active bacterial communities in arable soils.

2. Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing.

3. Comparison of 16S rRNA and 16S rDNA T-RFLP approaches to study bacterial communities in soil microcosms treated with chromate as perturbing agent.

4. A molecular approach to identify active microbes in environmental eukaryote clone libraries.

5. 16S rRNA sequences of uncultivated hot spring cyanobacterial mat inhabitants retrieved as randomly primed cDNA.

6. Amplification, cloning, and sequencing of a nifH segment from aquatic microorganisms and natural communities.

7. A practical optical trap for manipulating and isolating bacteria from complex microbial communities.

Review 8. Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

Review 9. A natural view of microbial biodiversity within hot spring cyanobacterial mat communities.

10. Direct 5S rRNA Assay for Monitoring Mixed-Culture Bioprocesses.