Literature DB >> 16347785

Purification and Characterization of Thermostable Pullulanase from Bacillus stearothermophilus and Molecular Cloning and Expression of the Gene in Bacillus subtilis.

T Kuriki1, J H Park, S Okada, T Imanaka.   

Abstract

A thermostable pullulanase (alpha-dextrin 6-glucanohydrolase [EC 3.2.1.41]) from a newly isolated Bacillus stearothermophilus strain (TRS128) was purified and characterized. The enzyme hydrolyzed (1-->6)-alpha-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65 degrees C. About 90% of the enzyme activity was retained after treatment at 65 degrees C for 60 min. By using pTB522 as a vector plasmid, the pullulanase gene was cloned and expressed in Bacillus subtilis.

Entities:  

Year:  1988        PMID： 16347785      PMCID： PMC204393          DOI： 10.1128/aem.54.11.2881-2883.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  General Biochemical Characterization of Thermostable Pullulanase and Glucoamylase from Clostridium thermohydrosulfuricum.

Authors:  H H Hyun; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

2.  Cloning and Expression of Thermostable alpha-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis.

Authors:  S Aiba; K Kitai; T Imanaka
Journal:  Appl Environ Microbiol       Date:  1983-11       Impact factor: 4.792

3.  A cultural method for the detection of pullulan--degrading enzymes in bacteria and its application to the genus Bacillus.

Authors:  F J Morgan; K R Adams; F G Priest
Journal:  J Appl Bacteriol       Date:  1979-04

4.  Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes W70.

Authors:  N Katsuragi; N Takizawa; Y Murooka
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

5.  Molecular characterization of pullulanase from Aerobacter aerogenes.

Authors:  B Eisele; I R Rasched; K Wallenfels
Journal:  Eur J Biochem       Date:  1972-03-15

6.  An improved purification of cell-bound pullulanase from Aerobacter aerogenes.

Authors:  C Mercier; B M Frantz; W J Whelan
Journal:  Eur J Biochem       Date:  1972-03-15

7.  New type of pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis.

Authors:  T Kuriki; S Okada; T Imanaka
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

8.  Cloning of the debranching-enzyme gene from Thermoanaerobium brockii into Escherichia coli and Bacillus subtilis.

Authors:  R D Coleman; S S Yang; M P McAlister
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

9.  Active-site- and substrate-specificity of Thermoanaerobium Tok6-B1 pullulanase.

Authors:  A R Plant; R M Clemens; H W Morgan; R M Daniel
Journal:  Biochem J       Date:  1987-09-01       Impact factor: 3.857

10.  Effect of in vitro DNA rearrangement in the NH2-terminal region of the penicillinase gene from Bacillus licheniformis on the mode of expression in Bacillus subtilis.

Authors:  T Imanaka; T Himeno; S Aiba
Journal:  J Gen Microbiol       Date:  1985-07
  10 in total
  9 in total

1.  A new way of producing isomalto-oligosaccharide syrup by using the transglycosylation reaction of neopullulanase.

Authors:  T Kuriki; M Yanase; H Takata; Y Takesada; T Imanaka; S Okada
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

2.  Characteristics of two forms of alpha-amylases and structural implication.

Authors:  K Ohdan; T Kuriki; H Kaneko; J Shimada; T Takada; Z Fujimoto; H Mizuno; S Okada
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

3.  Modes of action of acarbose hydrolysis and transglycosylation catalyzed by a thermostable maltogenic amylase, the gene for which was cloned from a Thermus strain.

Authors:  T J Kim; M J Kim; B C Kim; J C Kim; T K Cheong; J W Kim; K H Park
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

4.  Characterization of a gene cluster for glycogen biosynthesis and a heterotetrameric ADP-glucose pyrophosphorylase from Bacillus stearothermophilus.

Authors:  H Takata; T Takaha; S Okada; M Takagi; T Imanaka
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

5.  Characterization of a cold-adapted debranching enzyme and its role in glycogen metabolism and virulence of Vibrio vulnificus MO6-24/O.

Authors:  Ah-Reum Han; Haeyoung Kim; Jong-Tae Park; Jung-Wan Kim
Journal:  J Microbiol       Date:  2022-02-14       Impact factor: 2.902

6.  Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli.

Authors:  A Rüdiger; P L Jorgensen; G Antranikian
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

7.  Cloning of the aapT gene and characterization of its product, alpha-amylase-pullulanase (AapT), from thermophilic and alkaliphilic Bacillus sp. strain XAL601.

Authors:  S P Lee; M Morikawa; M Takagi; T Imanaka
Journal:  Appl Environ Microbiol       Date:  1994-10       Impact factor: 4.792

8.  Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance.

Authors:  Young-Wan Kim; Ji-Hye Choi; Jung-Wan Kim; Cheonseok Park; Jung-Woo Kim; Hyunju Cha; Soo-Bok Lee; Byoung-Ha Oh; Tae-Wha Moon; Kwan-Hwa Park
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

9.  Pullulanase: role in starch hydrolysis and potential industrial applications.

Authors:  Siew Ling Hii; Joo Shun Tan; Tau Chuan Ling; Arbakariya Bin Ariff
Journal:  Enzyme Res       Date:  2012-09-06
  9 in total

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