Literature DB >> 16347698

Consequences of accounting for isotopic dilution in thymidine incorporation assays.

T H Chrzanowski1.   

Abstract

Rates of thymidine incorporation into DNA were corrected for isotope dilution by internal nucleotide pools and were compared with rates obtained from uncorrected data. Differences as large as 109% were observed between corrected and uncorrected estimates of thymidine incorporation. The degree of underestimation varied seasonally and, to a lesser extent, spatially.

Entities:  

Year:  1988        PMID： 16347698      PMCID： PMC202761          DOI： 10.1128/aem.54.7.1868-1870.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Measurements of diel rates of bacterial secondary production in aquatic environments.

Authors:  B Riemann; M Søndergaard
Journal:  Appl Environ Microbiol       Date:  1984-04       Impact factor: 4.792

2.  Impact of storms on heterotrophic activity of epilimnetic bacteria in a southwestern reservoir.

Authors:  J G Hubbard; T H Chrzanowski
Journal:  Appl Environ Microbiol       Date:  1986-06       Impact factor: 4.792

3.  Further Verification of the Isotope Dilution Approach for Estimating the Degree of Participation of [H]thymidine in DNA Synthesis in Studies of Aquatic Bacterial Production.

Authors:  R T Bell
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

4.  Estimating Bacterioplankton Production by Measuring [H]thymidine Incorporation in a Eutrophic Swedish Lake.

Authors:  R T Bell; G M Ahlgren; I Ahlgren
Journal:  Appl Environ Microbiol       Date:  1983-06       Impact factor: 4.792

5.  Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california.

Authors:  J A Fuhrman; F Azam
Journal:  Appl Environ Microbiol       Date:  1980-06       Impact factor: 4.792

Review 6.  Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats.

Authors:  J T Staley; A Konopka
Journal:  Annu Rev Microbiol       Date:  1985       Impact factor: 15.500

7.  Validity of the tritiated thymidine method for estimating bacterial growth rates: measurement of isotope dilution during DNA synthesis.

Authors:  P C Pollard; D J Moriarty
Journal:  Appl Environ Microbiol       Date:  1984-12       Impact factor: 4.792
  7 in total
  6 in total

1.  Assessment of [h]thymidine incorporation into DNA as a method to determine bacterial productivity in stream bed sediments.

Authors:  L A Kaplan; T L Bott; J K Bielicki
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

2.  Underestimation of DNA synthesis by [h]thymidine incorporation in marine bacteria.

Authors:  W H Jeffrey; J H Paul
Journal:  Appl Environ Microbiol       Date:  1988-12       Impact factor: 4.792

3.  A Study on the lack of [methyl-(3)H] thymidine uptake and incorporation by chemolithotrophic bacteria.

Authors:  B H Johnstone; R D Jones
Journal:  Microb Ecol       Date:  1989-07       Impact factor: 4.552

4.  Catabolism of tritiated thymidine by aquatic microbial communities and incorporation of tritium into RNA and protein.

Authors:  A M Brittain; D M Karl
Journal:  Appl Environ Microbiol       Date:  1990-05       Impact factor: 4.792

5.  Adaptation of the 3H-leucine incorporation technique to measure heterotrophic activity associated with biofilm on the blades of the seaweed Sargassum spp.

Authors:  Sergio A Coelho-Souza; Marcio R Miranda; Leonardo T Salgado; Ricardo Coutinho; Jean R D Guimaraes
Journal:  Microb Ecol       Date:  2012-09-11       Impact factor: 4.552

6.  Estimates of bacterial growth rate constants from thymidine incorporation and variable conversion factors.

Authors:  T H Chrzanowski; K Simek; R H Sada; S Williams
Journal:  Microb Ecol       Date:  1993-03       Impact factor: 4.552
  6 in total

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