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Literature DB >> 16346825

High Production of Thermostable beta-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis.

Abstract

By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, beta-galactosidase is suitable for application in industrial processes.

Entities: Species

Year: 1985 PMID： 16346825 PMCID： PMC241766 DOI： 10.1128/aem.49.6.1547-1549.1985

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

8 in total

1. beta-Galactosidase from Bacillus stearothermophilus.

Authors: R E Goodman; D M Pederson
Journal: Can J Microbiol Date: 1976-06 Impact factor: 2.419

2. Properties of a thermostable beta-galactosidase from a thermophilic Bacillus: comparison of the enzyme activity of whole cells, purified enzyme and immobilised whole cells.

Authors: M W Griffiths; D D Muir
Journal: J Sci Food Agric Date: 1978-09 Impact factor: 3.638

3. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors: G R CRAVEN; E STEERS; C B ANFINSEN
Journal: J Biol Chem Date: 1965-06 Impact factor: 5.157

4. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB.

Authors: H Hirata; S Negoro; H Okada
Journal: J Bacteriol Date: 1984-10 Impact factor: 3.490

7. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

Authors: T J Gryczan; S Contente; D Dubnau
Journal: J Bacteriol Date: 1978-04 Impact factor: 3.490

8. Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product.

Authors: T Tanaka; N Kawano; T Oshima
Journal: J Biochem Date: 1981-02 Impact factor: 3.387

8 in total

13 in total

1. Biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA.

Authors: J M Coombs; J E Brenchley
Journal: Appl Environ Microbiol Date: 1999-12 Impact factor: 4.792

2. Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis.

Authors: Marcus Kaltwasser; Thomas Wiegert; Wolfgang Schumann
Journal: Appl Environ Microbiol Date: 2002-05 Impact factor: 4.792

3. Regulation of the Bacillus subtilis heat shock gene htpG is under positive control.

Authors: Saskia Versteeg; Angelika Escher; Andy Wende; Thomas Wiegert; Wolfgang Schumann
Journal: J Bacteriol Date: 2003-01 Impact factor: 3.490

Review 4. Enzyme stabilization: state of the art.

Authors: L Gianfreda; M R Scarfi
Journal: Mol Cell Biochem Date: 1991-02-02 Impact factor: 3.396

5. Heterogeneous properties of individual molecules of beta-galactosidase from the thermophilic bacteria Geobacillus stearothermophilus.

Authors: Douglas B Craig
Journal: Protein J Date: 2010-01 Impact factor: 2.371

9. Regulation of groE expression in Bacillus subtilis: the involvement of the sigma A-like promoter and the roles of the inverted repeat sequence (CIRCE).

Authors: G Yuan; S L Wong
Journal: J Bacteriol Date: 1995-10 Impact factor: 3.490

10. The htpG gene of Bacillus subtilis belongs to class III heat shock genes and is under negative control.

Authors: A Schulz; S Schwab; G Homuth; S Versteeg; W Schumann
Journal: J Bacteriol Date: 1997-05 Impact factor: 3.490