Literature DB >> 16346657

Properties of Malolactic Activity Purified from Leuconostoc oenos ML34 by Affinity Chromatography.

P Spettoli1, M P Nuti, A Zamorani.   

Abstract

Malolactic activity from Leuconostoc oenos ML34 is tightly associated with lactic dehydrogenase. A simple and fast procedure, involving affinity chromatography on agarose-hexane-NAD (Agnad), was used to separate malolactic activity from lactic dehydrogenase and other proteins. The yield was ca. 86%, the purification was 5.2-fold, and the K(m) values for l-malate, NAD and Mn were 2.8, 0.13, and 0.028 mM, respectively, at a pH optimum of 5.8.

Entities:  

Year:  1984        PMID： 16346657      PMCID： PMC241643          DOI： 10.1128/aem.48.4.900-901.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Biosynthesis of dicarboxylic acids by carbon dioxide fixation. V. Further study of the "malic" enzyme of Lactobacillus arabinosus.

Authors:  S KAUFMAN; S KORKES; A DEL CAMPILLO
Journal:  J Biol Chem       Date:  1951-09       Impact factor: 5.157

2.  Biosynthesis of dicarboxylic acids by carbon dioxide fixation. IV. Isolation and properties of an adaptive "malic" enzyme from Lactobacillus arabinosus.

Authors:  S KORKES; A DEL CAMPILLO; S OCHOA
Journal:  J Biol Chem       Date:  1950-12       Impact factor: 5.157

3.  Purification and Properties of a Malolactic Enzyme from a Strain of Leuconostoc mesenteroides Isolated from Grapes.

Authors:  A Lonvaud-Funel; A M de Saad
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Malolactic enzyme of Lactobacillus plantarum. Purification, properties, and distribution among bacteria.

Authors:  G Caspritz; F Radler
Journal:  J Biol Chem       Date:  1983-04-25       Impact factor: 5.157

6.  The synthesis of three AMP-analogues: N6-(6-aminohexyl)-adenosine 5'-monophosphate, N6-(6-aminohexyl)-adenosine 2',5'-bisphosphate, and N6-(6-aminohexyl)-adenosine 3',5'-bisphosphate and their application as general ligands in biospecific affinity chromatography.

Authors:  P Brodelius; P O Larsson; K Mosbach
Journal:  Eur J Biochem       Date:  1974-08-15
  6 in total
  7 in total

1.  Selection of Streptococcus lactis Mutants Defective in Malolactic Fermentation.

Authors:  P P Renault; H Heslot
Journal:  Appl Environ Microbiol       Date:  1987-02       Impact factor: 4.792

2.  Electrogenic malate uptake and improved growth energetics of the malolactic bacterium Leuconostoc oenos grown on glucose-malate mixtures.

Authors:  P Loubiere; P Salou; M J Leroy; N D Lindley; A Pareilleux
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

3.  Characterization of Leuconostoc oenos Isolated from Oregon Wines.

Authors:  Y S Izuagbe; T P Dohman; W E Sandine; D A Heatherbell
Journal:  Appl Environ Microbiol       Date:  1985-09       Impact factor: 4.792

4.  Kinetics, stereospecificity, and expression of the malolactic enzyme.

Authors:  C E Arthurs; D Lloyd
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

5.  Assessment of the genetic polymorphism and physiological characterization of indigenous Oenococcus oeni strains isolated from Aglianico del Vulture red wine.

Authors:  Caterina Cafaro; Maria Grazia Bonomo; Antonio Guerrieri; Fabiana Crispo; Rosanna Ciriello; Giovanni Salzano
Journal:  Folia Microbiol (Praha)       Date:  2015-05-28       Impact factor: 2.099

6.  Characterisation of putative lactate synthetic pathways of Coxiella burnetii.

Authors:  Janine Hofmann; Mebratu A Bitew; Miku Kuba; David P De Souza; Hayley J Newton; Fiona M Sansom
Journal:  PLoS One       Date:  2021-08-13       Impact factor: 3.240

7.  l-Malate (-2) Protonation State is Required for Efficient Decarboxylation to l-Lactate by the Malolactic Enzyme of Oenococcus oeni.

Authors:  Waldo Acevedo; Pablo Cañón; Felipe Gómez-Alvear; Jaime Huerta; Daniel Aguayo; Eduardo Agosin
Journal:  Molecules       Date:  2020-07-28       Impact factor: 4.411
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.