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Literature DB >> 1634444

Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells.

Abstract

Aedes albopictus mosquito cells resistant to the thymidine analog 5-bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 micrograms BrdU/ml, and for TK-8 cells was 45 micrograms/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 microM and a Vmax of 10 pmol.min-1.mg-1 protein.

Entities: Chemical Species

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Year: 1992 PMID： 1634444 DOI： 10.1007/bf02634051

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

18 in total

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Authors: H EAGLE
Journal: Science Date: 1959-08-21 Impact factor: 47.728

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Authors: M W McBurney; G F Whimore
Journal: J Cell Physiol Date: 1975-02 Impact factor: 6.384

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Authors: J A Lewis
Journal: Mol Cell Biol Date: 1986-06 Impact factor: 4.272

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Authors: N Nouri; A M Fallon
Journal: In Vitro Cell Dev Biol Date: 1987-03

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Authors: M Perucho; D Hanahan; L Lipsich; M Wigler
Journal: Nature Date: 1980-05-22 Impact factor: 49.962

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Authors: S J Mento; V Stollar
Journal: Somatic Cell Genet Date: 1978-03

7. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.

Authors: P F Lin; H B Lieberman; D B Yeh; T Xu; S Y Zhao; F H Ruddle
Journal: Mol Cell Biol Date: 1985-11 Impact factor: 4.272

8. Nucleoside and nucleobase transport and metabolism in wild type and nucleoside transport-deficient Aedes albopictus cells.

Authors: T F Abidi; P G Plagemann; C Woffendin; V Stollar
Journal: Biochim Biophys Acta Date: 1987-03-12

Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells.

1. Amino acid metabolism in mammalian cell cultures.

2. Mutants of chinese hamster cells resistant to adenosine.

3. Structure and expression of the Chinese hamster thymidine kinase gene.

4. Pleiotropic changes in cycloheximide-resistant insect cell clones.

5. Isolation of the chicken thymidine kinase gene by plasmid rescue.

6. Isolation and partial characterization of drug-resistant Aedes albopictus cells.

7. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.

8. Nucleoside and nucleobase transport and metabolism in wild type and nucleoside transport-deficient Aedes albopictus cells.

9. Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport.

10. Plaque formation and isolation of pure lines with poliomyelitis viruses.

1. Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB.

2. The oxidizing agent, paraquat, is more toxic to Wolbachia than to mosquito host cells.