Literature DB >> 6246445

Isolation of the chicken thymidine kinase gene by plasmid rescue.

M Perucho, D Hanahan, L Lipsich, M Wigler.   

Abstract

We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.

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Year:  1980        PMID： 6246445     DOI： 10.1038/285207a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  80 in total

1.  DNA-mediated transfer of an RNA polymerase II gene: reversion of the temperature-sensitive hamster cell cycle mutant TsAF8 by mammalian DNA.

Authors:  C J Ingles; M Shales
Journal:  Mol Cell Biol       Date:  1982-06       Impact factor: 4.272

2.  Sequences of transgene insertion sites in transgenic F4 common carp.

Authors:  Bo Wu; Yong-Hua Sun; Ya-Ping Wang; Yan-Wu Wang; Zuo-Yan Zhu
Journal:  Transgenic Res       Date:  2004-04       Impact factor: 2.788

3.  Haemophilus influenzae immunoglobulin A1 protease genes: cloning by plasmid integration-excision, comparative analyses, and localization of secretion determinants.

Authors:  F J Grundy; A Plaut; A Wright
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

4.  Binding of HMG 17 to mononucleosomes of the avian beta-globin gene cluster in erythroid and non-erythroid cells.

Authors:  T W Brotherton; J Reneker; G D Ginder
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

5.  Construction of microcell hybrid clones containing specific mouse chromosomes: application to autosomes 8 and 17.

Authors:  R E Fournier; J A Frelinger
Journal:  Mol Cell Biol       Date:  1982-05       Impact factor: 4.272

6.  High mutation frequency in DNA transfected into mammalian cells.

Authors:  M P Calos; J S Lebkowski; M R Botchan
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

7.  Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.

Authors:  P G Toye; N D MacHugh; A M Bensaid; S Alberti; A J Teale; W I Morrison
Journal:  Immunology       Date:  1990-05       Impact factor: 7.397

8.  Molecular cloning and cell cycle-specific regulation of a functional human thymidine kinase gene.

Authors:  H D Bradshaw
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

9.  Molecular cloning of the mouse ouabain-resistance gene.

Authors:  R Levenson; V Racaniello; L Albritton; D Housman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

10.  Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.

Authors:  Y F Lau; Y W Kan
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205
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