| Literature DB >> 16336940 |
Shen Luo1, Nancy B Wehr, Rodney L Levine.
Abstract
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to develop a rapid and sensitive method for detection and quantitation of proteins in gels and on blots. The fluorescence response is quantitative for protein content between 10 ng and 20 microg per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection.Entities:
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Year: 2005 PMID： 16336940 DOI： 10.1016/j.ab.2005.10.048
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365