Literature DB >> 16333116

Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.

Ryan R McDonald1, Nick A Antonishyn, Toni Hansen, Laelie A Snook, Evelyn Nagle, Michael R Mulvey, Paul N Levett, Greg B Horsman.   

Abstract

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

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Year:  2005        PMID： 16333116      PMCID： PMC1317231          DOI： 10.1128/JCM.43.12.6147-6149.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  A non-multiresistant community MRSA exposes its genome.

Authors:  Dlawer Ala'Aldeen
Journal:  Lancet       Date:  2002-05-25       Impact factor: 79.321

2.  Molecular epidemiology of community- and health care-associated methicillin-resistant Staphylococcus aureus in Manitoba, Canada.

Authors:  John L Wylie; Deborah L Nowicki
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

3.  Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT.

Authors:  S Narita; J Kaneko; J Chiba; Y Piémont; S Jarraud; J Etienne; Y Kamio
Journal:  Gene       Date:  2001-05-02       Impact factor: 3.688

4.  Panton-Valentine leucocidin and gamma-hemolysin from Staphylococcus aureus ATCC 49775 are encoded by distinct genetic loci and have different biological activities.

Authors:  G Prévost; B Cribier; P Couppié; P Petiau; G Supersac; V Finck-Barbançon; H Monteil; Y Piemont
Journal:  Infect Immun       Date:  1995-10       Impact factor: 3.441

Review 5.  Staphylococcus aureus: superbug, super genome?

Authors:  Jodi A Lindsay; Matthew T G Holden
Journal:  Trends Microbiol       Date:  2004-08       Impact factor: 17.079

6.  Detection of Panton-Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects.

Authors:  D Johnsson; P Mölling; K Strålin; B Söderquist
Journal:  Clin Microbiol Infect       Date:  2004-10       Impact factor: 8.067

7.  Management and outcome of children with skin and soft tissue abscesses caused by community-acquired methicillin-resistant Staphylococcus aureus.

Authors:  Michael C Lee; Ana M Rios; Monica Fonseca Aten; Asuncion Mejias; Dominick Cavuoti; George H McCracken; R Doug Hardy
Journal:  Pediatr Infect Dis J       Date:  2004-02       Impact factor: 2.129

Review 8.  Community-acquired methicillin-resistant Staphylococcus aureus: an emerging pathogen.

Authors:  Battouli Saïd-Salim; Barun Mathema; Barry N Kreiswirth
Journal:  Infect Control Hosp Epidemiol       Date:  2003-06       Impact factor: 3.254

9.  Community-associated methicillin-resistant Staphylococcus aureus, Canada.

Authors:  Michael R Mulvey; Laura MacDougall; Brenda Cholin; Greg Horsman; Melanie Fidyk; Shirley Woods
Journal:  Emerg Infect Dis       Date:  2005-06       Impact factor: 6.883

10.  Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence.

Authors:  Francois Vandenesch; Timothy Naimi; Mark C Enright; Gerard Lina; Graeme R Nimmo; Helen Heffernan; Nadia Liassine; Michèle Bes; Timothy Greenland; Marie-Elisabeth Reverdy; Jerome Etienne
Journal:  Emerg Infect Dis       Date:  2003-08       Impact factor: 6.883
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  34 in total

1.  A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens.

Authors:  Sabine Petersdorf; Miriam Herma; Meike Rosenblatt; Franziska Layer; Birgit Henrich
Journal:  J Clin Microbiol       Date:  2015-10-14       Impact factor: 5.948

2.  Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene.

Authors:  U Reischl; M J Tuohy; G S Hall; G W Procop; N Lehn; H Linde
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-02       Impact factor: 3.267

3.  Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci.

Authors:  Jo-Ann McClure; John M Conly; Vicky Lau; Sameer Elsayed; Thomas Louie; Wendy Hutchins; Kunyan Zhang
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

4.  Staphylococcus aureus isolated from endophthalmitis are hospital-acquired based on Panton-Valentine leukocidin and antibiotic susceptibility testing.

Authors:  Kristin A Rarey; Robert M Q Shanks; Eric G Romanowski; Francis S Mah; Regis P Kowalski
Journal:  J Ocul Pharmacol Ther       Date:  2011-10-20       Impact factor: 2.671

5.  Regional profiling for determination of genotype diversity of mastitis-specific Staphylococcus aureus lineage in Canada by use of clumping factor A, pulsed-field gel electrophoresis, and spa typing.

Authors:  Kamaleldin B Said; Johanne Ismail; Jennifer Campbell; Michael R Mulvey; Anne-Marie Bourgault; Serge Messier; Xin Zhao
Journal:  J Clin Microbiol       Date:  2009-12-02       Impact factor: 5.948

6.  Prevalence of toxin genes in consecutive clinical isolates of Staphylococcus aureus and clinical impact.

Authors:  T-X Nhan; R Leclercq; V Cattoir
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-01-12       Impact factor: 3.267

7.  Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp.

Authors:  Sukanya Sudhaharan; Lavanya Vanjari; Neeraja Mamidi; Nagapriyanka Ede; Lakshmi Vemu
Journal:  J Clin Diagn Res       Date:  2015-08-01

8.  Incidence, risk factors, and outcomes of Panton-Valentine leukocidin-positive methicillin-susceptible Staphylococcus aureus infections in Auckland, New Zealand.

Authors:  S Muttaiyah; G Coombs; S Pandey; P Reed; S Ritchie; D Lennon; S Roberts
Journal:  J Clin Microbiol       Date:  2010-08-04       Impact factor: 5.948

9.  New approaches to sepsis: molecular diagnostics and biomarkers.

Authors:  Konrad Reinhart; Michael Bauer; Niels C Riedemann; Christiane S Hartog
Journal:  Clin Microbiol Rev       Date:  2012-10       Impact factor: 26.132

10.  Detection of meticillin-resistant Staphylococcus aureus and Panton-Valentine leukocidin directly from clinical samples and the development of a multiplex assay using real-time polymerase chain reaction.

Authors:  L Renwick; A Hardie; E K Girvan; M Smith; G Leadbetter; E Claas; D Morrison; A P Gibb; J Dave; K E Templeton
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-03-21       Impact factor: 3.267
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