Literature DB >> 16333075

Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.

Oliver Goldenberg1, Stefanie Herrmann, Thomas Adam, Gina Marjoram, George Hong, Ulf B Göbel, Barbara Graf.   

Abstract

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

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Year:  2005        PMID： 16333075      PMCID： PMC1317168          DOI： 10.1128/JCM.43.12.5912-5915.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts.

Authors:  Y C Chen; J D Eisner; M M Kattar; S L Rassoulian-Barrett; K Lafe; U Bui; A P Limaye; B T Cookson
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.

Authors:  Eugen Domann; George Hong; Can Imirzalioglu; Simon Turschner; Johannes Kühle; Corinna Watzel; Torsten Hain; Hamid Hossain; Trinad Chakraborty
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

Review 3.  The use of denaturing high-performance liquid chromatography (DHPLC) for the analysis of genetic variations: impact for diagnostics and pharmacogenetics.

Authors:  Felix W Frueh; Mario Noyer-Weidner
Journal:  Clin Chem Lab Med       Date:  2003-04       Impact factor: 3.694

4.  Identification of clinically relevant yeasts by PCR/RFLP.

Authors:  Anja Trost; Barbara Graf; Jan Eucker; Orhan Sezer; Kurt Possinger; Ulf B Göbel; Thomas Adam
Journal:  J Microbiol Methods       Date:  2004-02       Impact factor: 2.363

5.  Trends in species causing fungaemia in a tertiary care medical centre over 12 years.

Authors:  P N Malani; S F Bradley; R S Little; C A Kauffman
Journal:  Mycoses       Date:  2001-12       Impact factor: 4.377

6.  Evaluation of the VITEK 2 system for rapid identification of yeasts and yeast-like organisms.

Authors:  B Graf; T Adam; E Zill; U B Göbel
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

7.  A one-year survey of candidemia in Belgium in 2002.

Authors:  D Swinne; M Watelle; C Suetens; K Mertens; P A Fonteyne; N Nolard
Journal:  Epidemiol Infect       Date:  2004-12       Impact factor: 2.451

8.  Prevalence of Candida species, including Candida dubliniensis, in Singapore.

Authors:  C W Yang; T M S Barkham; F Y Chan; Y Wang
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

9.  Denaturing HPLC for identifying bacteria.

Authors:  William Hurtle; David Shoemaker; Erik Henchal; David Norwood
Journal:  Biotechniques       Date:  2002-08       Impact factor: 1.993

10.  Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2).

Authors:  Thierry De Baere; Geert Claeys; Danielle Swinne; Gerda Verschraegen; An Muylaert; Caroline Massonet; Mario Vaneechoutte
Journal:  BMC Microbiol       Date:  2002-07-25       Impact factor: 3.605
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  6 in total

Review 1.  Interspecies interactions within oral microbial communities.

Authors:  Howard K Kuramitsu; Xuesong He; Renate Lux; Maxwell H Anderson; Wenyuan Shi
Journal:  Microbiol Mol Biol Rev       Date:  2007-12       Impact factor: 11.056

2.  Dual-model analysis for improving the discrimination performance of human and nonhuman blood based on Raman spectroscopy.

Authors:  Haiyi Bian; Peng Wang; Ning Wang; Yubing Tian; Pengli Bai; Haowen Jiang; Jing Gao
Journal:  Biomed Opt Express       Date:  2018-07-05       Impact factor: 3.732

3.  Development of a denaturing high-performance liquid chromatography method for detection of protist parasites of metazoans.

Authors:  Christofer Troedsson; Richard F Lee; Vivica Stokes; Tina L Walters; Paolo Simonelli; Marc E Frischer
Journal:  Appl Environ Microbiol       Date:  2008-05-23       Impact factor: 4.792

4.  Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany.

Authors:  Birgitta Schweickert; Oliver Goldenberg; Elvira Richter; Ulf B Göbel; Annette Petrich; Petra Buchholz; Annette Moter
Journal:  Emerg Infect Dis       Date:  2008-09       Impact factor: 6.883

5.  Application of sequence-dependent electrophoresis fingerprinting in exploring biodiversity and population dynamics of human intestinal microbiota: what can be revealed?

Authors:  Geert Huys; Tom Vanhoutte; Peter Vandamme
Journal:  Interdiscip Perspect Infect Dis       Date:  2008-12-14

6.  Gut microbiota patterns associated with colonization of different Clostridium difficile ribotypes.

Authors:  Jure Skraban; Saso Dzeroski; Bernard Zenko; Domen Mongus; Simon Gangl; Maja Rupnik
Journal:  PLoS One       Date:  2013-02-28       Impact factor: 3.240
  6 in total

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