Literature DB >> 16332894

Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag.

Francesca Scaramozzino1, Ilona Estruch, Paola Rossolillo, Marco Terreni, Alessandra M Albertini.   

Abstract

A tag of three lysines alternating with three glycines was added to the C-terminal end of the beta chain of penicillin G acylase (PGA). This modification improved the immobilization efficiency of PGA on glyoxyl agarose and the catalytic properties of the PGA derivative, although it impaired the posttranslational steps of overexpressed protein maturation.

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Year:  2005        PMID： 16332894      PMCID： PMC1317310          DOI： 10.1128/AEM.71.12.8937-8940.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies.

Authors:  W B Alkema; C M Hensgens; E H Kroezinga; E de Vries; R Floris; J M van der Laan; B W Dijkstra; D B Janssen
Journal:  Protein Eng       Date:  2000-12

2.  Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of Cephamandole from Cephalosporin C.

Authors:  M Terreni; G Pagani; D Ubiali; R Fernández-Lafuente; C Mateo; J M Guisán
Journal:  Bioorg Med Chem Lett       Date:  2001-09-17       Impact factor: 2.823

3.  Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft.

Authors:  L Hewitt; V Kasche; K Lummer; R J Lewis; G N Murshudov; C S Verma; G G Dodson; K S Wilson
Journal:  J Mol Biol       Date:  2000-09-29       Impact factor: 5.469

4.  Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment.

Authors:  Olga Abian; Valeria Grazú; Juan Hermoso; Ramón González; José Luis García; Roberto Fernández-Lafuente; José Manuel Guisán
Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

5.  Improving the activity of immobilized subtilisin by site-specific attachment to surfaces.

Authors:  W Huang; J Wang; D Bhattacharyya; L G Bachas
Journal:  Anal Chem       Date:  1997-11-15       Impact factor: 6.986

6.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

7.  Quantitative characterization of the nucleophile reactivity in penicillin acylase-catalyzed acyl transfer reactions.

Authors:  Maxim I Youshko; Ghermes G Chilov; Tatyana A Shcherbakova; Vytas K Svedas
Journal:  Biochim Biophys Acta       Date:  2002-09-23

8.  The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase.

Authors:  Wynand B L Alkema; Anne-Jan Dijkhuis; Erik De Vries; Dick B Janssen
Journal:  Eur J Biochem       Date:  2002-04

9.  DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA.

Authors:  S Radoja; O Francetić; N Stojićević; I Morić; V Glisin; M Konstantinović
Journal:  Genet Anal       Date:  1999-12-15

10.  Ligand-induced conformational change in penicillin acylase.

Authors:  S H Done; J A Brannigan; P C Moody; R E Hubbard
Journal:  J Mol Biol       Date:  1998-11-27       Impact factor: 5.469
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  2 in total

1.  New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase.

Authors:  Davide A Cecchini; Immacolata Serra; Daniela Ubiali; Marco Terreni; Alessandra M Albertini
Journal:  BMC Biotechnol       Date:  2007-09-10       Impact factor: 2.563

2.  Modulation of the microenvironment surrounding the active site of penicillin G acylase immobilized on acrylic carriers improves the enzymatic synthesis of cephalosporins.

Authors:  Paolo Bonomi; Teodora Bavaro; Immacolata Serra; Auro Tagliani; Marco Terreni; Daniela Ubiali
Journal:  Molecules       Date:  2013-11-20       Impact factor: 4.411
  2 in total

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