Literature DB >> 163285

A radioisotopic assay for proline oxidase activity.

J M Phang, S J Downing, D L Valle, E M Kowaloff.   

Abstract

We developed a radioisotopic assay for proline oxidase in which product deltal-pyrroline-5-carboxylate-14-C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.

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Year:  1975        PMID： 163285

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  20 in total

1.  Overexpression of proline oxidase induces proline-dependent and mitochondria-mediated apoptosis.

Authors:  Chien-an A Hu; Steven P Donald; Jian Yu; Wei-Wen Lin; Zhihe Liu; Gary Steel; Cassandra Obie; David Valle; James M Phang
Journal:  Mol Cell Biochem       Date:  2006-07-28       Impact factor: 3.396

2.  A sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method.

Authors:  H Ravi Kumar; S Ananda; K S Devaraju; B M Prakash; S Sampath Kumar; S V Suresh Babu; N Ramachandraswamy; H P Puttaraju
Journal:  Indian J Clin Biochem       Date:  2009-09-16

3.  Regulation of proline oxidase activity by lactate.

Authors:  E M Kowaloff; J M Phang; A S Granger; S J Downing
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

4.  Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.

Authors:  Ying Peng; Sandra K Cooper; Yi Li; Jay M Mei; Shuwei Qiu; Gregory L Borchert; Steven P Donald; Hsiang-Fu Kung; James M Phang
Journal:  Invest Ophthalmol Vis Sci       Date:  2015-04       Impact factor: 4.799

5.  Proline fed to intact soybean plants influences acetylene reducing activity and content and metabolism of proline in bacteroids.

Authors:  Y Zhu; G Shearer; D H Kohl
Journal:  Plant Physiol       Date:  1992-03       Impact factor: 8.340

6.  Oxidation of proline by plant mitochondria.

Authors:  S F Boggess; D E Koeppe
Journal:  Plant Physiol       Date:  1978-07       Impact factor: 8.340

7.  Transfer of 1-pyrroline-5-carboxylate as oxidizing potential from hepatocytes to erythrocytes.

Authors:  C H Hagedorn; G C Yeh; J M Phang
Journal:  Biochem J       Date:  1982-01-15       Impact factor: 3.857

8.  Functional consequences of PRODH missense mutations.

Authors:  Hans-Ulrich Bender; Shlomo Almashanu; Gary Steel; Chien-An Hu; Wei-Wen Lin; Alecia Willis; Ann Pulver; David Valle
Journal:  Am J Hum Genet       Date:  2005-01-20       Impact factor: 11.025

9.  A novel function for hydroxyproline oxidase in apoptosis through generation of reactive oxygen species.

Authors:  Sandra K Cooper; Jui Pandhare; Steven P Donald; James M Phang
Journal:  J Biol Chem       Date:  2008-02-19       Impact factor: 5.157

10.  Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum.

Authors:  P F Straub; P H Reynolds; S Althomsons; V Mett; Y Zhu; G Shearer; D H Kohl
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792
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