Development- and age-associated expression pattern of peroxiredoxin 6, and its regulation in murine ocular lens.

Peroxiredoxin (PRDX) 6 is a unique member of the PRDX family. Its antioxidant and signaling properties are related to its expression level in cells. We studied development- and age-associated changes in PRDX6 expression in the murine lens. We also investigated the effects of dexamethasone (Dex), transforming growth factor-beta1 (TGF-beta1) and tumor necrosis factor-alfa (TNF-alpha) on PRDX6 expression. Expression levels of PRDX6 mRNA in whole lenses isolated from postnatal day (PD) 1- to 18-month-old mice, and the effects of Dex, TGF-beta1 and TNF-alpha on the expression of PRDX6 in lens epithelial cells (LECs), were monitored using real-time reverse transcriptase-polymerase chain reaction (PCR) or Western blot. Localization of PRDX6 was studied using in situ hybridization and immunohistochemistry. PRDX6 expression gradually increased in the lenses of 4-week- to 6-month-old mice and declined thereafter. In situ hybridization and immunohistochemistry revealed that PRDX6 was localized in the cytoplasm of LECs and in lens fibers. Intense PRDX6 staining was present in the whole lens on gestational days 14 and 18. The lenses of PD1 mice showed diminished nuclear fiber staining, while those of 4-week-old mice revealed lack of nuclear fiber staining but intense staining of the germinative zone. LECs treated with TNF-alpha or Dex showed higher PRDX6 expression, while TGF-beta1 down regulated expression. Thus, our results provide a topographic basis for understanding the role of PRDX6 in the lens.