| Literature DB >> 16288203 |
K Haller1, K V Kibler, T Kasai, Y-H Chi, J-M Peloponese, V S R K Yedavalli, K-T Jeang.
Abstract
The spindle assembly checkpoint (SAC) guards against chromosomal mis-segregation and the emergence of aneuploidy. SAC in higher eukaryotes includes at least 10 proteins including MAD1-3, BUB1-3, and Msp1. A long-standing observation has been that rodent cells are more tolerant of microtubule toxins than primate cells indicating that SAC function is more relaxed in the former than the latter. Here, we report on an unexpected functional difference between the rodent and human MAD1 component of the respective SAC. Ectopic expression of human MAD1 in mouse and hamster cells corrected a relaxed SAC to a more stringent form. Our findings posit MAD1 as a species-specific determinant which influences the stringency of cellular response to microtubule depolymerization and spindle damage.Entities:
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Year: 2006 PMID： 16288203 DOI： 10.1038/sj.onc.1209259
Source DB: PubMed Journal: Oncogene ISSN： 0950-9232 Impact factor: 9.867