Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Efficient primer strand extension beyond oxadiazole carboxamide nucleobases.

Literature DB >> 16287267

Efficient primer strand extension beyond oxadiazole carboxamide nucleobases.

Olga Adelfinskaya¹, Vishal C Nashine, Donald E Bergstrom, V Jo Davisson.

Abstract

Two oxadiazole carboxamide deoxyribonucleoside analogues are described that can be incorporated and efficiently extended by Taq DNA polymerase. The primer strand extension beyond oxadiazole nucleoside analogues occurs at rates similar to the values observed for the canonical Watson-Crick base pairs irrespective of the template nucleobase. These distinctive chemical effects in DNA polymerase extensions are attributed to the smaller size and unique electronic properties of the oxadiazole nucleobase.

Entities: Chemical

Mesh：

Substances：

Year: 2005 PMID： 16287267 DOI： 10.1021/ja054226j

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

Keyword Cloud
Cited

8 in total

1. An efficiently extended class of unnatural base pairs.

Authors: Aaron M Leconte; Shigeo Matsuda; Floyd E Romesberg
Journal: J Am Chem Soc Date: 2006-05-31 Impact factor: 15.419

2. Toward a designed, functioning genetic system with expanded-size base pairs: solution structure of the eight-base xDNA double helix.

Authors: Stephen R Lynch; Haibo Liu; Jianmin Gao; Eric T Kool
Journal: J Am Chem Soc Date: 2006-11-15 Impact factor: 15.419

Efficient primer strand extension beyond oxadiazole carboxamide nucleobases.

1. An efficiently extended class of unnatural base pairs.

2. Toward a designed, functioning genetic system with expanded-size base pairs: solution structure of the eight-base xDNA double helix.

3. Minor groove hydrogen bonds and the replication of unnatural base pairs.

4. Polymerase amplification, cloning, and gene expression of benzo-homologous "yDNA" base pairs.

5. Synthesis of 2-(1,2,4-oxadiazol-3-yl)quinazolin-4(3H)-ones from diaminoglyoxime-based nitrones.

6. Discovery, characterization, and optimization of an unnatural base pair for expansion of the genetic alphabet.

7. Substituent effects on the pairing and polymerase recognition of simple unnatural base pairs.

8. Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids.