Literature DB >> 1627646

A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells.

Y Ke1, M C Wilkinson, D G Fernig, J A Smith, P S Rudland, R Barraclough.   

Abstract

Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.

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Year:  1992        PMID： 1627646     DOI： 10.1016/0167-4781(92)90029-y

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Interactions of putative heparin-binding domains of basic fibroblast growth factor and its receptor, FGFR-1, with heparin using synthetic peptides.

Authors:  L Kinsella; H L Chen; J A Smith; P S Rudland; D G Fernig
Journal:  Glycoconj J       Date:  1998-04       Impact factor: 2.916

2.  Enhanced cell-cell contact stability and decreased N-cadherin-mediated migration upon fibroblast growth factor receptor-N-cadherin cross talk.

Authors:  Thao Nguyen; Laurence Duchesne; Gautham Hari Narayana Sankara Narayana; Nicole Boggetto; David D Fernig; Chandrashekhar Uttamrao Murade; Benoit Ladoux; René-Marc Mège
Journal:  Oncogene       Date:  2019-07-16       Impact factor: 9.867

3.  Fibroblast growth factor-2 binds to small heparin-derived oligosaccharides and stimulates a sustained phosphorylation of p42/44 mitogen-activated protein kinase and proliferation of rat mammary fibroblasts.

Authors:  Maryse Delehedde; Malcolm Lyon; John T Gallagher; Philip S Rudland; David G Fernig
Journal:  Biochem J       Date:  2002-08-15       Impact factor: 3.857

4.  Diversification of the structural determinants of fibroblast growth factor-heparin interactions: implications for binding specificity.

Authors:  Ruoyan Xu; Alessandro Ori; Timothy R Rudd; Katarzyna A Uniewicz; Yassir A Ahmed; Scott E Guimond; Mark A Skidmore; Giuliano Siligardi; Edwin A Yates; David G Fernig
Journal:  J Biol Chem       Date:  2012-09-27       Impact factor: 5.157

5.  Expression of a functional recombinant human basic fibroblast growth factor from transgenic rice seeds.

Authors:  Na An; Jiquan Ou; Daiming Jiang; Liping Zhang; Jingru Liu; Kai Fu; Ying Dai; Daichang Yang
Journal:  Int J Mol Sci       Date:  2013-02-07       Impact factor: 5.923

6.  Sulfated polysaccharides interact with fibroblast growth factors and protect from denaturation.

Authors:  Changye Sun; Mengxin Liu; Panwen Sun; Mingming Yang; Edwin A Yates; Zhikun Guo; David G Fernig
Journal:  FEBS Open Bio       Date:  2019-07-16       Impact factor: 2.693

7.  Photothermal raster image correlation spectroscopy of gold nanoparticles in solution and on live cells.

Authors:  D J Nieves; Y Li; D G Fernig; R Lévy
Journal:  R Soc Open Sci       Date:  2015-06-17       Impact factor: 2.963
  7 in total

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