Literature DB >> 16266678

A homogeneous, high-throughput fluorescence resonance energy transfer-based DNA polymerase assay.

Adam Shapiro1, Olga Rivin, Ning Gao, Laurel Hajec.   

Abstract

A homogeneous, fluorescence resonance energy transfer (FRET)-based DNA polymerase assay that is suitable for high-throughput screening for inhibitors, and can also be used for steady-state kinetic investigations, is described. The activity, kinetic mechanism, and processivity of the isolated alpha subunit of DNA polymerase III, the product of the dnaE gene, from the gram-negative pathogen Haemophilus influenzae were investigated using the FRET assay.

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Year:  2005        PMID： 16266678     DOI： 10.1016/j.ab.2005.09.023

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Parallel multiplicative target screening against divergent bacterial replicases: identification of specific inhibitors with broad spectrum potential.

Authors:  H Garry Dallmann; Oliver J Fackelmayer; Guy Tomer; Joe Chen; Anna Wiktor-Becker; Tracey Ferrara; Casey Pope; Marcos T Oliveira; Peter M J Burgers; Laurie S Kaguni; Charles S McHenry
Journal:  Biochemistry       Date:  2010-03-23       Impact factor: 3.162

2.  Breaking the rules: bacteria that use several DNA polymerase IIIs.

Authors:  Charles S McHenry
Journal:  EMBO Rep       Date:  2011-04-08       Impact factor: 8.807

3.  Nonclassical SNAPFL analogue as a Cy5 resonance energy transfer partner.

Authors:  Sung Hoon Kim; Jillian R Gunther; John A Katzenellenbogen
Journal:  Org Lett       Date:  2008-10-09       Impact factor: 6.005

4.  Kinetic characterization of exonuclease-deficient Staphylococcus aureus PolC, a C-family replicative DNA polymerase.

Authors:  Indrajit Lahiri; Purba Mukherjee; Janice D Pata
Journal:  PLoS One       Date:  2013-05-16       Impact factor: 3.240
  4 in total

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