Literature DB >> 16248607

Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase.

James M Turner1, James Graziano, Glen Spraggon, Peter G Schultz.   

Abstract

It has been recently shown that orthogonal tRNA/aminoacyl-tRNA synthetase pairs can be evolved to allow genetic incorporation of unnatural amino acids into proteins in both prokaryotes and eukaryotes. Here we describe the crystal structure of an evolved aminoacyl-tRNA synthetase that charges the unnatural amino acid p-acetylphenylalanine. Molecular recognition is due to altered hydrogen bonding and packing interactions with bound substrate that result from changes in both side-chain and backbone conformation.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16248607     DOI： 10.1021/ja0549042

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  9 in total

1.  An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.

Authors:  Douglas D Young; Travis S Young; Michael Jahnz; Insha Ahmad; Glen Spraggon; Peter G Schultz
Journal:  Biochemistry       Date:  2011-02-01       Impact factor: 3.162

2.  Incorporation of fluorotyrosines into ribonucleotide reductase using an evolved, polyspecific aminoacyl-tRNA synthetase.

Authors:  Ellen C Minnihan; Douglas D Young; Peter G Schultz; JoAnne Stubbe
Journal:  J Am Chem Soc       Date:  2011-09-21       Impact factor: 15.419

3.  Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.

Authors:  Benjamin M Hutchins; Stephanie A Kazane; Karin Staflin; Jane S Forsyth; Brunhilde Felding-Habermann; Peter G Schultz; Vaughn V Smider
Journal:  J Mol Biol       Date:  2011-01-13       Impact factor: 5.469

4.  A critical examination of Escherichia coli esterase activity.

Authors:  Alicja K Antonczak; Zuzana Simova; Eric M Tippmann
Journal:  J Biol Chem       Date:  2009-08-07       Impact factor: 5.157

5.  Engineering aminoacyl-tRNA synthetases for use in synthetic biology.

Authors:  Natalie Krahn; Jeffery M Tharp; Ana Crnković; Dieter Söll
Journal:  Enzymes       Date:  2020-09-08

6.  Structural plasticity of an aminoacyl-tRNA synthetase active site.

Authors:  James M Turner; James Graziano; Glen Spraggon; Peter G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-17       Impact factor: 11.205

7.  Structural basis of improved second-generation 3-nitro-tyrosine tRNA synthetases.

Authors:  Richard B Cooley; Jessica L Feldman; Camden M Driggers; Taylor A Bundy; Audrey L Stokes; P Andrew Karplus; Ryan A Mehl
Journal:  Biochemistry       Date:  2014-03-20       Impact factor: 3.162

8.  Crystal Structure of an Archaeal Tyrosyl-tRNA Synthetase Bound to Photocaged L-Tyrosine and Its Potential Application to Time-Resolved X-ray Crystallography.

Authors:  Toshiaki Hosaka; Kazushige Katsura; Yoshiko Ishizuka-Katsura; Kazuharu Hanada; Kaori Ito; Yuri Tomabechi; Mio Inoue; Ryogo Akasaka; Chie Takemoto; Mikako Shirouzu
Journal:  Int J Mol Sci       Date:  2022-09-08       Impact factor: 6.208

9.  Study of the Binding Energies between Unnatural Amino Acids and Engineered Orthogonal Tyrosyl-tRNA Synthetases.

Authors:  Wei Ren; Tan M Truong; Hui-wang Ai
Journal:  Sci Rep       Date:  2015-07-29       Impact factor: 4.379
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.