Literature DB >> 16247959

Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases.

Shiv Kumar1, Anup Sood, Jeffery Wegener, Patrick J Finn, Satyam Nampalli, John R Nelson, Anuradha Sekher, Paul Mitsis, John Macklin, Carl W Fuller.   

Abstract

A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.

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Year:  2005        PMID： 16247959     DOI： 10.1081/ncn-200059823

Source DB:  PubMed          Journal:  Nucleosides Nucleotides Nucleic Acids        ISSN： 1525-7770            Impact factor:   1.381


  11 in total

Review 1.  The challenges of sequencing by synthesis.

Authors:  Carl W Fuller; Lyle R Middendorf; Steven A Benner; George M Church; Timothy Harris; Xiaohua Huang; Stevan B Jovanovich; John R Nelson; Jeffery A Schloss; David C Schwartz; Dmitri V Vezenov
Journal:  Nat Biotechnol       Date:  2009-11-06       Impact factor: 54.908

Review 2.  Sequencing technologies - the next generation.

Authors:  Michael L Metzker
Journal:  Nat Rev Genet       Date:  2009-12-08       Impact factor: 53.242

3.  Ligand-dependent exponential amplification of self-replicating RNA enzymes.

Authors:  Charles Olea; Gerald F Joyce
Journal:  Methods Enzymol       Date:  2014-12-05       Impact factor: 1.600

4.  Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagged nucleotides on a nanopore array.

Authors:  Carl W Fuller; Shiv Kumar; Mintu Porel; Minchen Chien; Arek Bibillo; P Benjamin Stranges; Michael Dorwart; Chuanjuan Tao; Zengmin Li; Wenjing Guo; Shundi Shi; Daniel Korenblum; Andrew Trans; Anne Aguirre; Edward Liu; Eric T Harada; James Pollard; Ashwini Bhat; Cynthia Cech; Alexander Yang; Cleoma Arnold; Mirkó Palla; Jennifer Hovis; Roger Chen; Irina Morozova; Sergey Kalachikov; James J Russo; John J Kasianowicz; Randy Davis; Stefan Roever; George M Church; Jingyue Ju
Journal:  Proc Natl Acad Sci U S A       Date:  2016-04-18       Impact factor: 11.205

5.  Synthesis of α,δ-Disubstituted Tetraphosphates and Terminally-Functionalized Nucleoside Pentaphosphates.

Authors:  Scott M Shepard; Hyehwang Kim; Qing Xin Bang; Norah Alhokbany; Christopher C Cummins
Journal:  J Am Chem Soc       Date:  2020-12-29       Impact factor: 15.419

6.  Fluorogenic DNA sequencing in PDMS microreactors.

Authors:  Peter A Sims; William J Greenleaf; Haifeng Duan; X Sunney Xie
Journal:  Nat Methods       Date:  2011-06-12       Impact factor: 28.547

7.  Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide gamma-phosphate derivative.

Authors:  Connie J Hansen; Lydia Wu; Jeffrey D Fox; Bahram Arezi; Holly H Hogrefe
Journal:  Nucleic Acids Res       Date:  2010-11-09       Impact factor: 16.971

8.  PEG-labeled nucleotides and nanopore detection for single molecule DNA sequencing by synthesis.

Authors:  Shiv Kumar; Chuanjuan Tao; Minchen Chien; Brittney Hellner; Arvind Balijepalli; Joseph W F Robertson; Zengmin Li; James J Russo; Joseph E Reiner; John J Kasianowicz; Jingyue Ju
Journal:  Sci Rep       Date:  2012-09-21       Impact factor: 4.379

9.  Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides.

Authors:  Jonas Korlach; Arek Bibillo; Jeffrey Wegener; Paul Peluso; Thang T Pham; Insil Park; Sonya Clark; Geoff A Otto; Stephen W Turner
Journal:  Nucleosides Nucleotides Nucleic Acids       Date:  2008-09       Impact factor: 1.381

Review 10.  DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present.

Authors:  Cheng-Yao Chen
Journal:  Front Microbiol       Date:  2014-06-24       Impact factor: 5.640
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