OBJECTIVE: To determine the prevalence of rhinovirus infection in hospitalized young infants with acute bronchiolitis. METHODS: Hospitalized children with acute bronchiolitis admitted to the Hospital São Lucas/PUCRS between May and September 2002 were selected prospectively. Nasopharyngeal samples were assayed for respiratory syncytial virus, parainfluenza, influenza and adenovirus by immunofluorescence. For rhinovirus test a reverse transcription-polymerase chain reaction for picornavirus was used, followed by hybridization with rhinovirus specific probes. RESULTS: Forty-five patients were selected for the study. The median age of the subjects studied was 2 months. Positive samples for respiratory viruses were found in 35/45 (77.8%) subjects and 7/35 (20%) patients had dual infection. Respiratory syncytial virus was detected in 33/35 (94%) cases. Rhinovirus was detected in 6/35 patients (17%). CONCLUSIONS: Rhinovirus was the second most common agent detected in nasal secretions from young infants hospitalized with acute bronchiolitis.
OBJECTIVE: To determine the prevalence of rhinovirus infection in hospitalized young infants with acute bronchiolitis. METHODS: Hospitalized children with acute bronchiolitis admitted to the Hospital São Lucas/PUCRS between May and September 2002 were selected prospectively. Nasopharyngeal samples were assayed for respiratory syncytial virus, parainfluenza, influenza and adenovirus by immunofluorescence. For rhinovirus test a reverse transcription-polymerase chain reaction for picornavirus was used, followed by hybridization with rhinovirus specific probes. RESULTS: Forty-five patients were selected for the study. The median age of the subjects studied was 2 months. Positive samples for respiratory viruses were found in 35/45 (77.8%) subjects and 7/35 (20%) patients had dual infection. Respiratory syncytial virus was detected in 33/35 (94%) cases. Rhinovirus was detected in 6/35 patients (17%). CONCLUSIONS: Rhinovirus was the second most common agent detected in nasal secretions from young infants hospitalized with acute bronchiolitis.
Authors: Josefina Garcia; Victoria Espejo; Martha Nelson; Merly Sovero; Manuel V Villaran; Jorge Gomez; Melvin Barrantes; Felix Sanchez; Guillermo Comach; Ana E Arango; Nicolas Aguayo; Ivette L de Rivera; Wilson Chicaiza; Mirna Jimenez; Washington Aleman; Francisco Rodriguez; Marina S Gonzales; Tadeusz J Kochel; Eric S Halsey Journal: Virol J Date: 2013-10-11 Impact factor: 4.099
Authors: Emerson Rodrigues da Silva; Márcio Condessa Paulo Pitrez; Eurico Arruda; Rita Mattiello; Edgar E Sarria; Flávia Escremim de Paula; José Luis Proença-Modena; Luana Sella Delcaro; Otávio Cintra; Marcus H Jones; José Dirceu Ribeiro; Renato T Stein Journal: BMC Infect Dis Date: 2013-01-25 Impact factor: 3.090