Literature DB >> 1624442

Transformation of the gram-positive bacterium Clavibacter xyli subsp. cynodontis by electroporation with plasmids from the IncP incompatibility group.

M C Metzler1, Y P Zhang, T A Chen.   

Abstract

We report the transformation of a gram-positive bacterium, Clavibacter xyli subsp. cynodontis, with several plasmids in the IncP incompatibility group from gram-negative bacteria. Our results suggest that IncP plasmids may be transferable to other gram-positive organisms. After optimizing electroporation parameters, we obtained a maximum of 2 x 10(5) transformants per microgram of DNA. The availability of a transformation system for this bacteria will facilitate its use in indirectly expressing beneficial traits in plants.

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Year:  1992        PMID： 1624442      PMCID： PMC206238          DOI： 10.1128/jb.174.13.4500-4503.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

1.  Ratoon stunting disease of sugarcane: isolation of the causal bacterium.

Authors:  M J Davis; A G Gillaspie; R W Harris; R H Lawson
Journal:  Science       Date:  1980-12-19       Impact factor: 47.728

2.  Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea.

Authors:  B Staskawicz; D Dahlbeck; N Keen; C Napoli
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

3.  Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression.

Authors:  G Ditta; T Schmidhauser; E Yakobson; P Lu; X W Liang; D R Finlay; D Guiney; D R Helinski
Journal:  Plasmid       Date:  1985-03       Impact factor: 3.466

4.  Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.

Authors:  N T Keen; S Tamaki; D Kobayashi; D Trollinger
Journal:  Gene       Date:  1988-10-15       Impact factor: 3.688

5.  Transformation of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and Escherichia coli with the C. diphtheriae plasmid pNG2.

Authors:  T M Serwold-Davis; N Groman; M Rabin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

6.  A host-vector system for an Arthrobacter species.

Authors:  P C Shaw; B S Hartley
Journal:  J Gen Microbiol       Date:  1988-04

7.  Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector.

Authors:  D Meletzus; R Eichenlaub
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

8.  Improved electroporation and cloning vector system for gram-positive bacteria.

Authors:  G M Dunny; L N Lee; D J LeBlanc
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

9.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

10.  Intergeneric conjugation between Escherichia coli and Streptomyces species.

Authors:  P Mazodier; R Petter; C Thompson
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490
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  10 in total

Review 1.  Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum.

Authors:  Marco Ventura; Carlos Canchaya; Andreas Tauch; Govind Chandra; Gerald F Fitzgerald; Keith F Chater; Douwe van Sinderen
Journal:  Microbiol Mol Biol Rev       Date:  2007-09       Impact factor: 11.056

2.  Integrative Cloning, Expression, and Stability of the cryIA(c) Gene from Bacillus thuringiensis subsp. kurstaki in a Recombinant Strain of Clavibacter xyli subsp. cynodontis.

Authors:  J S Lampel; G L Canter; M B Dimock; J L Kelly; J J Anderson; B B Uratani; J S Foulke; J T Turner
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

3.  A Type Ib ParB protein involved in plasmid partitioning in a gram-positive bacterium.

Authors:  Ping Yin; Tai-Yuan Li; Mao-Hua Xie; Lina Jiang; Yi Zhang
Journal:  J Bacteriol       Date:  2006-09-22       Impact factor: 3.490

4.  Gene targeting in gram-negative bacteria by use of a mobile group II intron ("Targetron") expressed from a broad-host-range vector.

Authors:  Jun Yao; Alan M Lambowitz
Journal:  Appl Environ Microbiol       Date:  2007-02-23       Impact factor: 4.792

5.  Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon.

Authors:  J M Blatny; T Brautaset; H C Winther-Larsen; K Haugan; S Valla
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

6.  Characterization of the variants, flanking genes, and promoter activity of the Leifsonia xyli subsp. cynodontis insertion sequence IS1237.

Authors:  Hui Lin; Tai-Yuan Li; Mao-Hua Xie; Yi Zhang
Journal:  J Bacteriol       Date:  2007-02-09       Impact factor: 3.490

7.  Transfer of the broad-host-range IncQ plasmid RSF1010 and other plasmid vectors to the gram-positive methylotroph Brevibacterium methylicum by electrotransformation.

Authors:  J Nesvera; J Hochmannová; M Pátek; A Sroglová; V Becvárová
Journal:  Appl Microbiol Biotechnol       Date:  1994-02       Impact factor: 4.813

8.  Stable transformation of the gram-positive phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus with several cloning vectors.

Authors:  M J Laine; H Nakhei; J Dreier; K Lehtilä; D Meletzus; R Eichenlaub; M C Metzler
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792

9.  Characterization of new IS elements and studies of their dispersion in two subspecies of Leifsonia xyli.

Authors:  Marcelo M Zerillo; Marie-Anne Van Sluys; Luis Eduardo A Camargo; Claudia B Monteiro-Vitorello
Journal:  BMC Microbiol       Date:  2008-07-25       Impact factor: 3.605

10.  Comparative genome analysis of pathogenic and non-pathogenic Clavibacter strains reveals adaptations to their lifestyle.

Authors:  Joanna Załuga; Pieter Stragier; Steve Baeyen; Annelies Haegeman; Johan Van Vaerenbergh; Martine Maes; Paul De Vos
Journal:  BMC Genomics       Date:  2014-05-22       Impact factor: 3.969
  10 in total

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