Literature DB >> 16244131

The Kluyveromyces lactis gamma-toxin targets tRNA anticodons.

Jian Lu1, Bo Huang, Anders Esberg, Marcus J O Johansson, Anders S Byström.   

Abstract

Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.

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Year:  2005        PMID： 16244131      PMCID： PMC1370851          DOI： 10.1261/rna.2172105

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  32 in total

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Authors:  A R Butler; J H White; Y Folawiyo; A Edlin; D Gardiner; M J Stark
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  111 in total

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Review 4.  tRNA biology charges to the front.

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Authors:  Jo Marie Bacusmo; Silvia S Orsini; Jennifer Hu; Michael DeMott; Patrick C Thiaville; Ameer Elfarash; Mellie June Paulines; Diego Rojas-Benítez; Birthe Meineke; Chris Deutsch; Dirk Iwata-Reuyl; Patrick A Limbach; Peter C Dedon; Kelly C Rice; Stewart Shuman; Valérie de Crécy-Lagard
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9.  Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA.

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10.  Molecular basis of bacterial protein Hen1 activating the ligase activity of bacterial protein Pnkp for RNA repair.

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