Literature DB >> 16242860

Loop-mediated isothermal amplification for the rapid detection of Salmonella.

Yukiko Hara-Kudo1, Manabu Yoshino, Tadashi Kojima, Masanari Ikedo.   

Abstract

Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.

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Year:  2005        PMID： 16242860     DOI： 10.1016/j.femsle.2005.09.032

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  47 in total

1.  Sensitive and rapid detection of the new Delhi metallo-beta-lactamase gene by loop-mediated isothermal amplification.

Authors:  Wei Liu; Dayang Zou; Yan Li; Xuesong Wang; Xiang He; Xiao Wei; Changlin Shao; Xuelian Li; Wei Shang; Kaitao Yu; Dawei Liu; Yunmei Li; Jing Guo; Zhitao Yin; Jing Yuan
Journal:  J Clin Microbiol       Date:  2012-02-22       Impact factor: 5.948

2.  Large Fragment of DNA Polymerase I from Geobacillus sp. 777: Cloning and Comparison with DNA Polymerases I in Practical Applications.

Authors:  Igor P Oscorbin; Ulyana A Boyarskikh; Maksim L Filipenko
Journal:  Mol Biotechnol       Date:  2015-10       Impact factor: 2.695

3.  Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.

Authors:  Siyi Chen; Fei Wang; John C Beaulieu; Rebecca E Stein; Beilei Ge
Journal:  Appl Environ Microbiol       Date:  2011-04-15       Impact factor: 4.792

4.  Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.

Authors:  Mohammadali Safavieh; Manoj K Kanakasabapathy; Farhang Tarlan; Minhaz U Ahmed; Mohammed Zourob; Waseem Asghar; Hadi Shafiee
Journal:  ACS Biomater Sci Eng       Date:  2016-01-21

5.  Biomolecular and structural analyses of cauliflower-like DNAs by ultraviolet, circular dichroism, and fluorescence spectroscopies in comparison with natural DNA.

Authors:  Pooria Gill; Bijan Ranjbar; Reza Saber; Khosro Khajeh; Mehdi Mohammadian
Journal:  J Biomol Tech       Date:  2011-07

6.  Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment.

Authors:  Lu Han; Kaidi Wang; Lina Ma; Pascal Delaquis; Susan Bach; Jinsong Feng; Xiaonan Lu
Journal:  Appl Environ Microbiol       Date:  2020-03-18       Impact factor: 4.792

7.  Isolation and drug susceptibility of Candida parapsilosis sensu lato and other species of C. parapsilosis complex from patients with blood stream infections and proposal of a novel LAMP identification method for the species.

Authors:  Plinio Trabasso; Tetsuhiro Matsuzawa; Renata Fagnani; Yasunori Muraosa; Kenichiro Tominaga; Mariangela Ribeiro Resende; Katsuhiko Kamei; Yuzuru Mikami; Angelica Zaninelli Schreiber; Maria Luiza Moretti
Journal:  Mycopathologia       Date:  2014-12-07       Impact factor: 2.574

8.  Development of a toxR-based loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus.

Authors:  Siyi Chen; Beilei Ge
Journal:  BMC Microbiol       Date:  2010-02-10       Impact factor: 3.605

9.  Efficient identification of clinically relevant Candida yeast species by use of an assay combining panfungal loop-mediated isothermal DNA amplification with hybridization to species-specific oligonucleotide probes.

Authors:  João Inácio; Orfeu Flores; Isabel Spencer-Martins
Journal:  J Clin Microbiol       Date:  2007-12-12       Impact factor: 5.948

10.  Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis.

Authors:  Narisara Chantratita; Ella Meumann; Aunchalee Thanwisai; Direk Limmathurotsakul; Vanaporn Wuthiekanun; Saran Wannapasni; Sarinna Tumapa; Nicholas P J Day; Sharon J Peacock
Journal:  J Clin Microbiol       Date:  2007-11-26       Impact factor: 5.948
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