S Ninawe1, R C Kuhad. 1. Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, India. sninawe@dbt.nic.in
Abstract
AIM: The present study aimed at optimization of cultural and nutritional parameters for enhanced production of xylanase from Streptomyces cyaneus SN32. METHODS AND RESULTS: The xylanase production by S. cyaneus SN32 on most of the agro-residues tested in this study was more, as compared with the xylanase yield in the medium supplemented with commercial xylan. The presence of wheat bran as carbon source in the medium induced the highest production of xylanase followed by corn cob. Utilization of maize stalk, gram husk and black gram husk for microbial xylanase production has been reported first time in the present study. Among all the organic and inorganic sources of nitrogen tested in the study, peptone was found to be the best in stimulating xylanase production by S. cyaneus SN32. CONCLUSION: The production of xylanase from this thermoalkalophilic actinomycete has been enhanced 1.44-fold. To the best of our knowledge, the magnitude of enzyme yield i.e. 720 IU ml(-1) by S. cyaneus SN32 has not been reported for any other actinomycete so far. SIGNIFICANCE AND IMPACT OF STUDY: Present studies revealed that thermoalkalophilic S. cyaneus SN32, because of its simple nutritional requirements and its ability to exhibit considerably good enzyme yield, is a potent xylanase producer for its economical application in various industries.
AIM: The present study aimed at optimization of cultural and nutritional parameters for enhanced production of xylanase from Streptomyces cyaneus SN32. METHODS AND RESULTS: The xylanase production by S. cyaneus SN32 on most of the agro-residues tested in this study was more, as compared with the xylanase yield in the medium supplemented with commercial xylan. The presence of wheat bran as carbon source in the medium induced the highest production of xylanase followed by corn cob. Utilization of maize stalk, gram husk and black gramhusk for microbial xylanase production has been reported first time in the present study. Among all the organic and inorganic sources of nitrogen tested in the study, peptone was found to be the best in stimulating xylanase production by S. cyaneus SN32. CONCLUSION: The production of xylanase from this thermoalkalophilic actinomycete has been enhanced 1.44-fold. To the best of our knowledge, the magnitude of enzyme yield i.e. 720 IU ml(-1) by S. cyaneus SN32 has not been reported for any other actinomycete so far. SIGNIFICANCE AND IMPACT OF STUDY: Present studies revealed that thermoalkalophilic S. cyaneus SN32, because of its simple nutritional requirements and its ability to exhibit considerably good enzyme yield, is a potent xylanase producer for its economical application in various industries.