Biosynthesis and regulation of microbial polyunsaturated fatty acid production.

Growing interest in polyunsaturated fatty acid (PUFA) applications in various fields coupled with their significance in health and dietary requirements has focused attention on the provision of suitable sources of these compounds. Isolation of highly efficient oleaginous microorganisms has led to the development of fermentation technologies as an alternative to agricultural and animal processes. Particularly active in PUFA synthesis are the Zygomycetes fungi and certain microalgae. Emphasis is placed on increasing the product value by employing new biotechnological strategies (e.g. mutation techniques, molecular engineering and biotransformations) which allow the regulation of microbial PUFA formation with satisfactory yield in order to be competitive with other sources. Comparative successes in fungal PUFA production demonstrate microbial potential to synthesize high-value oils and provide the main stimulus for their applications.