Literature DB >> 1623197

Construction of in-frame chimeric plant viral genes by simplified PCR strategies.

C D Atreya1, P L Atreya, T P Pirone.   

Abstract

The use of polymerase chain reaction (PCR)-based mutagenesis to create chimeric genes is presently not cost-effective because of the size and number of primers as well as the number of PCRs required. We have developed two strategies based on inverse PCR that exploit limited homologies between two DNA molecules to create in-frame chimeric plant viral genes. This report also contains a compilation of information useful for determining possible restriction sites at a given common dipeptide coding motif between any genes of interest.

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Year:  1992        PMID： 1623197     DOI： 10.1007/bf00023403

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  10 in total

1.  Selection of deletion mutants by polymerase chain reaction.

Authors:  C D Atreya; P L Atreya; T P Pirone
Journal:  Biochem Biophys Res Commun       Date:  1990-12-31       Impact factor: 3.575

Review 2.  A biochemical perspective of the polymerase chain reaction.

Authors:  W Bloch
Journal:  Biochemistry       Date:  1991-03-19       Impact factor: 3.162

3.  A general method of polymerase-chain-reaction-enabled protein domain mutagenesis: construction of a human protein S-osteonectin gene.

Authors:  X C Villarreal; G L Long
Journal:  Anal Biochem       Date:  1991-09-02       Impact factor: 3.365

4.  The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein.

Authors:  R Allison; R E Johnston; W G Dougherty
Journal:  Virology       Date:  1986-10-15       Impact factor: 3.616

5.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

6.  'Sticky feet'-directed mutagenesis and its application to swapping antibody domains.

Authors:  T Clackson; G Winter
Journal:  Nucleic Acids Res       Date:  1989-12-25       Impact factor: 16.971

7.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

Authors:  R M Horton; H D Hunt; S N Ho; J K Pullen; L R Pease
Journal:  Gene       Date:  1989-04-15       Impact factor: 3.688

8.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

Authors:  J M Clark
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

9.  The nucleotide sequence of tobacco vein mottling virus RNA.

Authors:  L L Domier; K M Franklin; M Shahabuddin; G M Hellmann; J H Overmeyer; S T Hiremath; M F Siaw; G P Lomonossoff; J G Shaw; R E Rhoads
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

10.  Construction of recombinant DNA molecules by the use of a single stranded DNA generated by the polymerase chain reaction: its application to chimeric hepatitis A virus/poliovirus subgenomic cDNA.

Authors:  C Wychowski; S U Emerson; J Silver; S M Feinstone
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971
  10 in total
  1 in total

1.  Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility.

Authors:  C D Atreya; T P Pirone
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-15       Impact factor: 11.205
  1 in total

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