Literature DB >> 16227502

Spectral unmixing of flavin autofluorescence components in cardiac myocytes.

D Chorvat1, J Kirchnerova, M Cagalinec, J Smolka, A Mateasik, A Chorvatova.   

Abstract

We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.

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Year:  2005        PMID： 16227502      PMCID： PMC1367005          DOI： 10.1529/biophysj.105.073866

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  7 in total

1.  Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals.

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Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

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Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-17       Impact factor: 11.205

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Authors:  W S Kunz; F N Gellerich
Journal:  Biochem Med Metab Biol       Date:  1993-08

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Authors:  W S Kunz; W Kunz
Journal:  Biochim Biophys Acta       Date:  1985-09-06

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Authors:  C L Hall
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

6.  Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein.

Authors:  Shaohui Huang; Ahmed A Heikal; Watt W Webb
Journal:  Biophys J       Date:  2002-05       Impact factor: 4.033

7.  Quantitative NAD(P)H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response.

Authors:  Jonathan V Rocheleau; W Steven Head; David W Piston
Journal:  J Biol Chem       Date:  2004-05-17       Impact factor: 5.157
  7 in total
  19 in total

1.  Permeabilized rat cardiomyocyte response demonstrates intracellular origin of diffusion obstacles.

Authors:  Natalja Jepihhina; Nathalie Beraud; Mervi Sepp; Rikke Birkedal; Marko Vendelin
Journal:  Biophys J       Date:  2011-11-01       Impact factor: 4.033

2.  Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes.

Authors:  Filip Sedlic; Danijel Pravdic; Naoyuki Hirata; Yasushi Mio; Ana Sepac; Amadou K Camara; Tetsuro Wakatsuki; Zeljko J Bosnjak; Martin Bienengraeber
Journal:  Biochim Biophys Acta       Date:  2010-07-17

3.  Spectral unmixing of multicolored bioluminescence emitted from heterogeneous biological sources.

Authors:  Seth T Gammon; W Matthew Leevy; Shimon Gross; George W Gokel; David Piwnica-Worms
Journal:  Anal Chem       Date:  2006-03-01       Impact factor: 6.986

4.  Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues.

Authors:  Jonathan A Palero; Henriëtte S de Bruijn; Angélique van der Ploeg van den Heuvel; Henricus J C M Sterenborg; Hans C Gerritsen
Journal:  Biophys J       Date:  2007-04-20       Impact factor: 4.033

5.  Rapid changes in NADH and flavin autofluorescence in rat cardiac trabeculae reveal large mitochondrial complex II reserve capacity.

Authors:  Rob C I Wüst; Michiel Helmes; Ger J M Stienen
Journal:  J Physiol       Date:  2015-03-13       Impact factor: 5.182

6.  Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection.

Authors:  Kwan Seob Park; Dong Uk Kim; Jooran Lee; Geon Hee Kim; Ki Soo Chang
Journal:  Biomed Opt Express       Date:  2016-05-19       Impact factor: 3.732

Review 7.  A theoretical-experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods.

Authors:  Silas J Leavesley; Brenner Sweat; Caitlyn Abbott; Peter Favreau; Thomas C Rich
Journal:  J Biophotonics       Date:  2017-05-09       Impact factor: 3.207

8.  Hyperspectral imaging fluorescence excitation scanning for colon cancer detection.

Authors:  Silas J Leavesley; Mikayla Walters; Carmen Lopez; Thomas Baker; Peter F Favreau; Thomas C Rich; Paul F Rider; Carole W Boudreaux
Journal:  J Biomed Opt       Date:  2016-10-01       Impact factor: 3.170

9.  Spectrally resolved time-correlated single photon counting: a novel approach for characterization of endogenous fluorescence in isolated cardiac myocytes.

Authors:  D Chorvat; A Chorvatova
Journal:  Eur Biophys J       Date:  2006-10-11       Impact factor: 1.733

10.  PROBING THE IMPACT OF GAMMA-IRRADIATION ON THE METABOLIC STATE OF NEURAL STEM AND PRECURSOR CELLS USING DUAL-WAVELENGTH INTRINSIC SIGNAL TWO-PHOTON EXCITED FLUORESCENCE.

Authors:  Tatiana B Krasieva; Erich Giedzinski; Katherine Tran; Mary Lan; Charles L Limoli; Bruce J Tromberg
Journal:  J Innov Opt Health Sci       Date:  2011-07-01
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