| Literature DB >> 16223481 |
Carley N Sauter1, Rebecca L McDermid, Amy L Weinberg, Tamara L Greco, Xiaojie Xu, Fern E Murdoch, Michael K Fritsch.
Abstract
The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERalpha, ERbeta) are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen-dependent manner by endogenous ERs or by transiently overexpressed ERalpha. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation.Entities:
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Year: 2005 PMID： 16223481 PMCID： PMC1350973 DOI： 10.1016/j.yexcr.2005.09.005
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905