| Literature DB >> 1621955 |
R P Alderton1, L M Eccleston, R P Howe, C A Read, M A Reeve, S Beck.
Abstract
A method for the preparation of multiple M13 DNA sequencing templates is described. No phenol extraction is required and the procedure can be carried out in Eppendorf tubes or 96-well microtiter plates. Starting with a phage supernatant, the entire procedure is carried out in the same reaction vessel and all separation steps are based on a novel application of magnetic bead separation. The design of a 96-well magnetic separator is presented and the application of the method for large-scale sequencing is discussed.Entities:
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Year: 1992 PMID： 1621955 DOI： 10.1016/0003-2697(92)90190-i
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365