Eisuke Uchino1, Shozo Sonoda, Kumiko Nakao, Taiji Sakamoto. 1. Department of Ophthalmology, Faculty of Medicine, Kagoshima University Graduate School of Medicine and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
Abstract
PURPOSE: To study the effects of eye closure on the balance of tear cytokines. METHODS: A total of 17 healthy volunteers with normal eyes were recruited. Tears (10 microl) were collected by capillary outflow at 09:00. The right eyes were closed with cotton bandages for 3 h, then tears were collected from each eye separately. The concentrations of inflammatory cytokines, interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12p70, and tumor necrosis factor (TNF)-alpha were measured by cytometric bead array. RESULTS: Although the concentration of each cytokine varied between eyes, the increasing ratio for 3 h showed a similar pattern in each cytokine. IL-1beta, IL-6, IL-10, IL-12p70, and TNF-alpha increased and IL-8 decreased in normal eyes. After eye closure, the increase of IL-10 significantly diminished (P=0.037); however, the other cytokines were not affected. CONCLUSIONS: Eye closure decreased the concentration of IL-10 in tears, which might cause a potentially pro-inflammatory status of the ocular surface.
PURPOSE: To study the effects of eye closure on the balance of tear cytokines. METHODS: A total of 17 healthy volunteers with normal eyes were recruited. Tears (10 microl) were collected by capillary outflow at 09:00. The right eyes were closed with cotton bandages for 3 h, then tears were collected from each eye separately. The concentrations of inflammatory cytokines, interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12p70, and tumor necrosis factor (TNF)-alpha were measured by cytometric bead array. RESULTS: Although the concentration of each cytokine varied between eyes, the increasing ratio for 3 h showed a similar pattern in each cytokine. IL-1beta, IL-6, IL-10, IL-12p70, and TNF-alpha increased and IL-8 decreased in normal eyes. After eye closure, the increase of IL-10 significantly diminished (P=0.037); however, the other cytokines were not affected. CONCLUSIONS: Eye closure decreased the concentration of IL-10 in tears, which might cause a potentially pro-inflammatory status of the ocular surface.
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